Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Biliary immunoreactive trypsin in man.

G Lake-Bakaar, J Dooley

    Hepato-Gastroenterology
    |June 1, 1987
    PubMed
    Summary
    This summary is machine-generated.

    Bile contains small amounts of trypsin/trypsinogen (IRT), even with bile duct obstruction. Proteolytic enzyme inhibitors in bile are significantly lower than IRT, suggesting a unique role in the biliary system.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Qualitative study of clinician and patient perspectives on the mode of anaesthesia for emergency surgery.

    The British journal of surgery·2019
    Same author

    Moral reasoning and decision-making in adolescents who sustain traumatic brain injury.

    Brain injury·2018
    Same author

    Evidence for long-term sensitization of the bowel in patients with post-infectious-IBS.

    Scientific reports·2017
    Same author

    An orthologous non-MHC locus in rats and mice is linked to CD4<sup>+</sup> and CD8<sup>+</sup> T-cell proportion.

    Genes and immunity·2017
    Same author

    Programmed cell death-1 expression correlates with disease severity and IL-5 in chronic rhinosinusitis with nasal polyps.

    Allergy·2017
    Same author

    Hamstring/Quadriceps Strength Ratios in Collegiate Middle-Distance and Distance Runners.

    The Physician and sportsmedicine·2016
    Same journal

    Palliative Surgery for Gastric Cancer with Gastric Outlet Obstruction or Anemia due to Tumor Bleeding.

    Hepato-gastroenterology·2016
    Same journal

    A Novel "Artery First" Approach Allowing Safe Resection in Laparoscopic Pancreaticoduodenectomy: The Uncinate Process First Approach.

    Hepato-gastroenterology·2016
    Same journal

    A Phase I Study of S-1 and Gemcitabine with Concurrent Radiotherapy in Patients with Non-Metastatic Advanced Pancreatic Cancer.

    Hepato-gastroenterology·2016
    Same journal

    A Novel Approach for Endoscopic Papillary Balloon Dilation with the Guidewire Left in the Pancreatic Duct to Ensure Pancreatic Stenting.

    Hepato-gastroenterology·2016
    Same journal

    Which Factors are Predictive for Mortality among Hospitalized Patients with Cirrhosis?

    Hepato-gastroenterology·2016
    Same journal

    Hydrodynamics Analysis and CFD Simulation of Portal Venous System by TIPS and LS.

    Hepato-gastroenterology·2016
    See all related articles

    Area of Science:

    • Gastroenterology
    • Biochemistry
    • Enzymology

    Background:

    • Proteolytic enzymes like trypsin/trypsinogen (IRT) are crucial in digestion.
    • Bile duct obstruction can alter the composition of bile.
    • The presence and role of enzymes and inhibitors in bile are not fully understood.

    Purpose of the Study:

    • To investigate the presence and levels of immunoreactive trypsin/trypsinogen (IRT) in bile.
    • To quantify major serum proteolytic enzyme inhibitors in bile.
    • To compare the bile-to-serum ratios of IRT and its inhibitors.

    Main Methods:

    • Percutaneous transhepatic catheterization to obtain bile samples from patients with complete distal bile duct obstruction.
    • Measurement of immunoreactive trypsin/trypsinogen (IRT) in bile.

    Related Experiment Videos

  • Quantification of alpha 2-macroglobulin and alpha 1-antitrypsin in bile and serum.

    • Main Results:

    • Significant amounts of IRT were detected in bile, uncontaminated by pancreatic juice.
    • Very small quantities of alpha 2-macroglobulin and alpha 1-antitrypsin were found in bile.
    • Bile-to-serum ratios for these inhibitors were substantially lower than that for IRT.

    Conclusions:

    • IRT is present in bile even during complete bile duct obstruction.
    • Major serum protease inhibitors are present in low concentrations in bile.
    • The specific role and significance of these inhibitors in biliary proteolytic enzyme regulation remain to be elucidated.