Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein-protein Interfaces02:04

Protein-protein Interfaces

14.7K
Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
14.7K
Protein-Protein Interfaces02:04

Protein-Protein Interfaces

4.5K
4.5K
The Nucleosome Core Particle02:10

The Nucleosome Core Particle

14.5K
Nucleosomes are the DNA-histone complex, where the DNA strand is wound around the histone core. The histone core is an octamer containing two copies of H2A, H2B, H3, and H4 histone proteins.
The paradox
Nucleosomes, paradoxically, perform two opposite functions simultaneously. On the one hand, their main responsibility is to protect the delicate DNA strands from physical damage and help achieve a higher compaction ratio. While on the other hand, they must allow polymerase enzymes to access DNA...
14.5K
Viral Structure00:56

Viral Structure

74.5K
Viruses are extraordinarily diverse in shape and size, but they all have several structural features in common. All viruses have a core that contains a DNA- or RNA-based genome. The core is surrounded by a protective coat of proteins called the capsid. The capsid is composed of subunits called capsomeres. The capsid and genome-containing core are together known as the nucleocapsid.
74.5K
What are Viruses?00:50

What are Viruses?

128.2K
Overview
128.2K
Retroviruses02:33

Retroviruses

15.0K
Retroviruses and retrotransposons both insert copies of their genetic elements into the genome of the host cell. Thus, the viral genes are passed on when the host genome is replicated or translated. A typical retroviral DNA sequence contains 3-4 genes that encode the different proteins required for its structural assembly and function as a molecular parasite. This DNA is transcribed into a single mRNA, which is very similar in structure to conventional mRNAs, i.e., it is capped at the 5’...
15.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Gut Microbiota Drives Aging-related Erythropoiesis Impairment via Phenylacetic Acid-induced Histone Phenylacetylation.

Blood·2026
Same author

Errate: Limb-Salvage Outcomes of Arterial Repair Beyond Time Limit at Different Lower-Extremity Injury Sites.

Medical science monitor : international medical journal of experimental and clinical research·2026
Same author

Targeted Delivery of Indole-3-Pyruvic Acid Suppresses Macrophage Ferroptosis to Enhance CD8<sup>+</sup> T Cell-Mediated Immunotherapy Response in Bladder Cancer.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

An H<sub>2</sub>O<sub>2</sub> and MPO programmable responsive MRI probe for early detection of drug-induced acute kidney injury via spatiotemporal monitoring of renal oxidative stress and inflammation.

Redox biology·2026
Same author

Active Hydrogen Reservoir Enabled by p-d Orbital Hybridization in PdSb Metallene for Electrocatalytic Alkynol Semi‑Hydrogenation at Large Current Densities.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

New insight into light utilization efficiency: an evaluation of semitransparent solar cells for building-integrated photovoltaic windows.

Scientific reports·2026

Related Experiment Video

Updated: Feb 3, 2026

A Protocol for Analyzing Hepatitis C Virus Replication
13:04

A Protocol for Analyzing Hepatitis C Virus Replication

Published on: June 26, 2014

24.7K

Hepatitis B virus core protein dimer‑dimer interface is critical for viral replication.

Chang-Long Zheng1, Yong-Mei Fu1, Zhan-Xue Xu2

  • 1Department of Emergency, The Third Affiliated Hospital of Sun Yat‑Sen University, Guangzhou, Guangdong 510630, P.R. China.

Molecular Medicine Reports
|November 3, 2018
PubMed
Summary

Mutations in the Hepatitis B virus (HBV) core protein dimer-dimer interface disrupt capsid assembly and HBV replication. This interface is crucial for viral function and may be a novel antiviral target.

Keywords:
hepatitis B viruscore proteindimer-dimer interfacecapsidreplicationvirion

More Related Videos

Chemical Dimerization-Induced Protein Condensates on Telomeres
08:52

Chemical Dimerization-Induced Protein Condensates on Telomeres

Published on: April 12, 2021

3.7K
Development of a Hepatitis B Virus Reporter System to Monitor the Early Stages of the Replication Cycle
09:35

Development of a Hepatitis B Virus Reporter System to Monitor the Early Stages of the Replication Cycle

Published on: February 1, 2017

14.0K

Related Experiment Videos

Last Updated: Feb 3, 2026

A Protocol for Analyzing Hepatitis C Virus Replication
13:04

A Protocol for Analyzing Hepatitis C Virus Replication

Published on: June 26, 2014

24.7K
Chemical Dimerization-Induced Protein Condensates on Telomeres
08:52

Chemical Dimerization-Induced Protein Condensates on Telomeres

Published on: April 12, 2021

3.7K
Development of a Hepatitis B Virus Reporter System to Monitor the Early Stages of the Replication Cycle
09:35

Development of a Hepatitis B Virus Reporter System to Monitor the Early Stages of the Replication Cycle

Published on: February 1, 2017

14.0K

Area of Science:

  • Virology
  • Molecular Biology
  • Structural Biology

Background:

  • Hepatitis B virus (HBV) core protein (HBc) is essential for viral capsid assembly, genome replication, and virion production.
  • HBc monomers form dimers, and these dimers assemble into icosahedral capsids via a dimer-dimer interface.

Purpose of the Study:

  • To investigate the role of the HBc dimer-dimer interface in HBV replication.
  • To identify key residues within the dimer-dimer interface essential for HBV assembly and replication.

Main Methods:

  • Site-directed mutagenesis of HBc residues at the dimer-dimer interface.
  • Native and denaturing gel electrophoresis, western blotting, Southern and Northern blotting, and quantitative PCR.
  • Analysis of capsid assembly, pregenomic RNA encapsidation, and viral DNA replication.

Main Results:

  • Mutations at the dimer-dimer interface, particularly at residues 23-39, disrupted capsid assembly.
  • None of the dimer-dimer interface mutants supported viral RNA encapsidation or DNA replication.
  • Mutants inhibited wild-type HBc genome replication and virion production but did not affect nuclear covalently closed circular DNA levels.

Conclusions:

  • The HBc dimer-dimer interface is critical for normal capsid function and HBV replication.
  • The HBc dimer-dimer interface represents a potential novel antiviral target for Hepatitis B therapy.