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Determination of vitamin D3 in liquid multivitamin preparation, using reverse phase, solid phase extraction liquid
Journal - Association of Official Analytical Chemists
|May 1, 1987
Summary
This study details a liquid chromatography method for accurately quantifying vitamin D3 in liquid multivitamins. The validated method ensures reliable vitamin D3 determination in commercial preparations.
Area of Science:
- Analytical Chemistry
- Biochemistry
- Nutritional Science
Background:
- Accurate quantification of vitamin D3 is crucial for nutritional supplements.
- Existing methods may face challenges with complex matrices like liquid multivitamins.
Purpose of the Study:
- To develop and validate a robust liquid chromatography method for vitamin D3 determination.
- To ensure accurate and reliable analysis of vitamin D3 in liquid multivitamin preparations.
Main Methods:
- Utilized reverse-phase solid-phase extraction (SPE) for sample purification.
- Employed liquid chromatography with a Zorbax ODS column and a specific solvent system.
- Detected vitamin D3 using a UV detector at 254 nm.
Main Results:
- Achieved complete resolution of vitamin D3 from interfering compounds within approximately 21 minutes.
- Demonstrated high accuracy with a mean recovery of 98.5% of theory.
- Showcased precision with a coefficient of variation of 3.8%.
Conclusions:
- The developed liquid chromatography method is effective for vitamin D3 quantification in liquid multivitamins.
- The method provides accurate and reproducible results for commercial preparations.
- This analytical approach supports quality control in the supplement industry.