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Updated: Feb 3, 2026
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Application of Retinoic Acid to Obtain Osteocytes Cultures from Primary Mouse Osteoblasts
Published on: May 13, 2014
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The controlled naringin release from TiO2 nanotubes to regulate osteoblast differentiation
Min Lai1, Ziyang Jin1, Mengying Yan1
11 School of Life Science, Jiangsu Normal University, Xuzhou, Jiangsu, China.
Journal of Biomaterials Applications
|November 3, 2018
Summary
Chitosan-coated titanium dioxide nanotubes loaded with naringin improve bone cell growth and function. This novel biomaterial enhances osteointegration for better bone implant integration.
Area of Science:
- Biomaterials Science
- Nanotechnology
- Biomedical Engineering
Background:
- Titanium (Ti)-based biomaterials are crucial for bone implants.
- Controlled drug release and bioactivity are desired properties for enhanced osteointegration.
- Titanium dioxide (TiO2) nanotubes offer a promising platform for drug delivery applications.
Purpose of the Study:
- To fabricate and characterize naringin-loaded TiO2 nanotubes coated with chitosan.
- To evaluate the in vitro drug release profile of naringin from the functionalized nanotubes.
- To investigate the biological effects of these modified nanotubes on osteoblast cells.
Main Methods:
- Fabrication of TiO2 nanotubes via electrochemical anodization.
- Loading of naringin and subsequent coating with chitosan.
- Characterization using field emission scanning electron microscopy, atomic force microscopy, X-ray photoelectron spectroscopy, and contact angle measurements.
- In vitro drug release studies using UV-visible spectrophotometry.
- In vitro assessment of osteoblast cell behavior (spreading, proliferation, alkaline phosphatase activity, mineralization).
Main Results:
- Successful fabrication of TiO2 nanotubes with controlled dimensions.
- Demonstrated controlled in vitro release of naringin.
- Chitosan-coated, naringin-loaded TiO2 nanotubes significantly enhanced osteoblast spreading, proliferation, and alkaline phosphatase activity.
- Improved late-stage osteoblast mineralization was observed with the modified nanotubes.
Conclusions:
- Chitosan-coated naringin-loaded TiO2 nanotubes exhibit excellent bioactive properties.
- These functionalized nanotubes promote osteoblast function and mineralization.
- This approach offers a promising strategy for developing advanced biomaterials to enhance bone-implant osteointegration in clinical settings.
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