Hyperthermophilic composting significantly decreases N₂O emissions by regulating N₂O-related functional genes

This study reported for the first time that hyperthermophilic composting (HTC) could mitigate 90% of the cumulative amount of N₂O emissions compared to traditional composting (TC) in a full-scale experiment. The concentrations of NO₂^--N and NO₃^--N in HTC were significantly lower than those in TC, which may be the main reason for the reduced N₂O emissions. Furthermore, this study found that the decrease in N₂O emissions in HTC compared to TC was mainly due to the inhibition of the abundance of the bacterial amoA and norB genes, which could decrease the nitrification rate and control N₂O formation, respectively. Partial least squares path modeling revealed that a high temperature was the key factor in lowering N₂O emissions in HTC, while physicochemical properties were the dominant factor in enhancing N₂O emissions in TC. These results suggested that HTC is a promising technique for reducing N₂O emissions in manure composting.