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Genotyping Mycoplasma synoviae: Development of a multi-locus variable number of tandem-repeats analysis and

Zsuzsa Kreizinger1, Kinga M Sulyok1, Katinka Bekő1

  • 1Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, H-1143, Budapest, Hungária krt. 21., Hungary.

Veterinary Microbiology
|November 4, 2018
PubMed

Insights

A new multi-locus variable number of tandem-repeats analysis (MLVA) method effectively types Mycoplasma synoviae strains. This rapid, cost-effective technique aids in controlling avian mycoplasmosis and distinguishing field strains from vaccines.

Area of Science:

  • Veterinary microbiology
  • Avian infectious diseases
  • Molecular epidemiology

Background:

  • Mycoplasma synoviae is a significant avian pathogen, challenging to control due to its transmission routes and global dissemination.
  • Molecular typing is crucial for Mycoplasma synoviae control programs and epidemiological studies.
  • Existing methods may have limitations in speed, cost, or applicability to clinical samples.

Purpose of the Study:

  • To develop a novel multi-locus variable number of tandem-repeats analysis (MLVA) method for Mycoplasma synoviae.
  • To evaluate the performance of the developed MLVA against established methods like multi-locus sequence typing (MLST).
  • To assess the utility of MLVA for differentiating field strains from live vaccine strains.

Main Methods:

  • Selection of seven tandem repeat (TR) regions from the Mycoplasma synoviae genome.
  • Application of the MLVA method to 86 Mycoplasma synoviae strains from 15 countries.
  • Comparative analysis with MLST, vlhA gene sequencing, and vaccine strain differentiation assays.

Main Results:

  • The developed MLVA method identified 35 distinct genotypes among the 86 strains, offering genetic resolution comparable to MLST.
  • MLVA successfully differentiated live vaccine strains from field strains.
  • The method demonstrated high discriminative power, speed, and cost-effectiveness.

Conclusions:

  • The developed MLVA is a powerful, efficient tool for the genetic characterization of Mycoplasma synoviae.
  • This method supports epidemiological surveillance and disease control efforts.
  • MLVA can complement existing assays, especially in ambiguous cases of vaccine strain differentiation.
Keywords:
DiscriminationMLSTMLVAMycoplasma synoviaeVaccine strainvlhA

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