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Visualization and Quantification of Brown and Beige Adipose Tissues in Mice using [18F]FDG Micro-PET/MR Imaging
Published on: July 1, 2021
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PET Imaging of PARP Expression Using 18F-Olaparib
Thomas C Wilson1, Mary-Ann Xavier2, James Knight2
1Department of Chemistry, University of Oxford, Oxford, United Kingdom; and.
Summary
We developed 18F-olaparib, a novel radiotracer for imaging Poly(ADP-ribose) polymerase (PARP) activity. This tracer shows promise for noninvasive tumor imaging and monitoring radiation damage in cancer patients.
Area of Science:
- Nuclear medicine
- Radiochemistry
- Cancer therapy
Background:
- Poly(ADP-ribose) polymerase (PARP) inhibitors are crucial in cancer treatment.
- Noninvasive imaging of PARP activity is needed for patient selection and therapy monitoring.
Purpose of the Study:
- To develop and evaluate 18F-olaparib, a novel PET imaging agent for PARP.
- To assess the potential of 18F-olaparib for monitoring radiation-induced DNA damage.
Main Methods:
- Copper-mediated 18F-radiofluorination of aryl boronic esters to synthesize 18F-olaparib.
- In vitro studies using PARP-1 expressing cells.
- PET imaging in mice bearing PARP-1 expressing tumors, with and without irradiation.
Main Results:
- 18F-olaparib selectively uptake in PARP-1 expressing cells.
- Tumor uptake of 18F-olaparib correlated with PARP-1 expression in vivo.
- Tumor uptake increased by 70% after irradiation, demonstrating sensitivity to DNA damage.
Conclusions:
- 18F-olaparib is a novel PET tracer for imaging PARP activity.
- 18F-olaparib enables noninvasive monitoring of radiation damage in tumors.
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