Transient and permanent arterial occlusions modeling poststroke epilepsy in aging rats
Eric R Miller1, Elena A Kharlamov2, Zeyu Hu3
1Neurology and Neuroscience Institute, Allegheny General Hospital, Allegheny Health Network, Pittsburgh, PA, 15212, United States.
Epilepsy Research
|November 5, 2018
Summary
This study compared permanent and transient middle cerebral artery occlusion (MCAO) models for inducing post-stroke epilepsy (PSE) in aging rats. Permanent MCAO showed promise for modeling PSE, despite high mortality rates.
Area of Science:
- Neuroscience
- Epileptology
- Stroke Research
Background:
- Post-stroke epilepsy (PSE) mechanisms in aged brains are poorly understood due to limited animal models.
- Previous work utilized transient middle cerebral artery (MCA) and common carotid artery (CCA) occlusion (MCA/CCAo) in rats.
- Age and infarction independently influence seizure frequency in existing models.
Purpose of the Study:
- To evaluate permanent unilateral MCA/CCAo as a simpler, comparable model for studying post-stroke epilepsy (PSE).
- To compare outcomes of permanent MCA/CCAo with transient MCA/CCAo in different aged Fischer (F344) rats.
Main Methods:
- Performed permanent and transient unilateral MCA/CCAo in 4-, 12-, and 20-month-old F344 rats.
- Monitored animals using video-electroencephalography (video-EEG) for seizure activity.
- Assessed infarct volumes and performed Timm staining for mossy fiber sprouting.
Main Results:
- High mortality (54%) observed, particularly in older animals and with permanent occlusion.
- Five animals (14%) exhibited epileptic seizure activity, including focal nonconvulsive and generalized convulsive seizures.
- No significant difference in infarct volumes between permanent and transient occlusion; no mossy fiber sprouting detected.
Conclusions:
- Permanent MCA/CCAo is a viable method for modeling post-stroke epilepsy (PSE) in younger aged rats (4- and 12-month-old).
- The model demonstrates focal electrographic ictal discharges and behavioral seizures.
- Further refinement is needed to address high mortality rates in older animals.
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