Effect of phenanthrene on the physicochemical properties of earthworm casts in soil

Earthworms have been widely studied as bioindicators of soil health for their important role in sustaining soil structure and functions. Many soil contaminants such as phenanthrene have been confirmed to exert adverse effects on earthworms' growth, reproduction, behaviors and biochemical conditions. However, their effects on the properties of earthworm casts have been little studied. In the present study, the effect of different doses of phenanthrene (PHE) (0, 2, 5, 10, 20 mg/kg) on the six physicochemical properties and Fourier transform infrared spectroscopy (FTIR) spectra characteristics of earthworm casts was assessed in artificial soil in a laboratory. 1) Residual concentration of PHE in soils and casts increased with the increasing exposure concentrations and followed the order of casts > soil, concluding that K_ow values are the important factor affecting the distribution of hydrophobic organic contaminants (HOCs) in soil and casts; 2) Earthworms produced casts with improved total organic carbon (TOC) (15-19%), NH₄⁺-N (550-800^%), total available phosphorus (TAP) (300-450%), cation exchange capacity (CEC) (about 15%) and available potassium (AK) (7-12.6%) compared to that in unpolluted soil, indicating that earthworms still have the ability to play the role of ecological engineers even in polluted soil; 3) The sensitivity of different properties of casts to phenanthrene varies, the order of sensitivity being (most sensitive first) NH₄⁺-N ( triggered as 2 mg/kg of exposure concentrations) > AK (5 mg/kg) > Olsen-P (10 mg/kg) > TOC = pH= CEC (no response within the range of exposure concentrations). NH₄⁺-N content in casts shows a clear dose-response relationship when the exposure exceeds 2 mg/kg, indicating that the index might be a potential sensitive biomarker to provide early warning for soil pollution. 4) FTIR spectra showed that the constitution of casts from earthworms in PHE-spiked soil was not significantly alternated. However, FTIR spectra revealed that the concentrations of C-O of polysaccharide in casts increased with the elevated exposure concentrations, indicating that intensities of C-O of polysaccharide at 1032 cm^-1 of casts might be also a potential biomarker for the early-warning of soil pollution. This study advances the knowledge of earthworm ecology in polluted soil, and further extends the scope of earthworm casts as a potential biomarker in soil pollution assessment.