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Sub-acute Cerebral Microhemorrhages Induced by Lipopolysaccharide Injection in Rats
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Sub-acute Cerebral Microhemorrhages Induced by Lipopolysaccharide Injection in Rats

Published on: October 17, 2018

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Sub-acute Cerebral Microhemorrhages Induced by Lipopolysaccharide Injection in Rats

Dandan Li1, Hóngyi Zhào2, Wei Wei3

  • 1Department of Neurology, Second Hospital of Shanxi Medical University; Department of Neurology, PLA Army General Hospital.

Journal of Visualized Experiments : Jove
|November 6, 2018
PubMed

Insights

This study presents a new rat model for cerebral microhemorrhages (CMHs) using lipopolysaccharide (LPS) to induce inflammation. The model allows for reliable detection of CMHs using various imaging and staining techniques.

Area of Science:

  • Neuroscience
  • Pathology
  • Animal Models

Background:

  • Cerebral microhemorrhages (CMHs) are prevalent in aging and linked to neuropsychiatric disorders.
  • Neuroinflammation is frequently associated with the complex causes of CMHs.

Purpose of the Study:

  • To establish a sub-acute rat model for cerebral microhemorrhages (CMHs).
  • To detail methods for detecting CMHs in this new animal model.
  • To compare this model with other existing CMH models.

Main Methods:

  • Induction of CMHs via systemic lipopolysaccharide (LPS) injection in rats.
  • Detection of CMHs using gross observation, HE staining, Perl's Prussian staining, Evans blue (EB) double-labeling.
  • Utilizing magnetic resonance imaging-susceptibility weighted imaging (MRI-SWI) for CMH detection.

Main Results:

  • Successfully developed a stable and cost-effective sub-acute CMH rat model.
  • Demonstrated multiple reliable methods for detecting CMHs induced by LPS.
  • Provided a comparative analysis of different CMH animal models.

Conclusions:

  • The LPS-induced rat model offers a viable method for studying CMHs and associated neuroinflammation.
  • The described detection techniques are effective for identifying CMHs in this model.
  • This model facilitates research into the etiology and treatment of CMHs.

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