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Updated: Feb 3, 2026
Isolation of Mouse Peritoneal Cavity Cells
Published on: January 28, 2010
The antibacterial effect of peritoneal fluid in experimental peritonitis
Birol Ağca1, Ahmet Yalın İşcan, Erdal Polat
1Department of General Surgery, University of Health Sciences, İstanbul Fatih Sultan Mehmet Training and Research Hospital, İstanbul-Turkey. birolagca@yahoo.com.
Background:
In our study, the effects of peritoneal fluid on some Gram-negative and Candida albicans in experimental peritonitis rats were studied. The primary objective of the present study was to understand the effect of peritoneal fluid on microorganisms causing intra-abdominal infections.
Methods:
Twenty male Sprague-Dawley rats weighing between 250 and 300 g were used in the study. The rats were randomly divided into two groups consisting of 10 animals. The operative procedures were performed under sterile conditions. In group I, sham laparotomy was done. In group II, the distal part of the cecum was ligated, and cecum perforation was performed. Peritoneal fluid samples at baseline and 2 and 4 h were extracted using a Pasteur pipette during laparotomy under anesthesia.
Results:
Peritoneal fluid was ineffective on Citrobacter freundii, Proteus mirabilis, and Enterobacter aerogenes. It inhibited the growth of Klebsiella pneumoniae for 8 h. However, growth was significantly increased in the passages obtained after 24 h. The growth of C. albicans decreased in the passages that were extracted after 4 and 8 h and increased in the passages obtained after 24 h (p<0.05). It was found that the number of Escherichia coli and Pseudomonas aeruginosa colonies that were grown in 2 h decreased, and no growth was detected in the passages obtained after 2 h (p<0.05).
Conclusion:
Proliferating colony counts of E. coli and P. aeruginosa decreased after 2 h, and there was no proliferation in subsequent cultures. Peritoneal fluid exhibits a bactericidal effect under appropriate conditions. It also exhibits peritoneal bactericidal activity against E. coli, the major pathogen in intra-abdominal infections.
Insights
Peritoneal fluid shows bactericidal effects against common intra-abdominal infection pathogens like Escherichia coli and Pseudomonas aeruginosa. This study investigated the impact of peritoneal fluid on various bacteria and Candida albicans in rats.
Area of Science:
- Microbiology
- Infectious Diseases
- Experimental Medicine
Background:
- Intra-abdominal infections (IAIs) pose significant clinical challenges.
- Understanding the host's innate immune response, specifically peritoneal fluid's antimicrobial properties, is crucial for developing effective treatments.
- This study investigates the antimicrobial activity of peritoneal fluid against key pathogens involved in IAIs.
Purpose of the Study:
- To evaluate the in vitro effect of rat peritoneal fluid on Gram-negative bacteria and Candida albicans.
- To determine the impact of peritoneal fluid on the growth and viability of microorganisms commonly found in experimental peritonitis models.
Main Methods:
- Twenty male Sprague-Dawley rats were used, divided into sham laparotomy and cecal perforation groups.
- Peritoneal fluid samples were collected at baseline, 2, and 4 hours post-procedure under sterile conditions.
- The antimicrobial activity of collected peritoneal fluid was assessed against Citrobacter freundii, Proteus mirabilis, Enterobacter aerogenes, Klebsiella pneumoniae, Escherichia coli, Pseudomonas aeruginosa, and Candida albicans.
Main Results:
- Peritoneal fluid demonstrated ineffectiveness against C. freundii, P. mirabilis, and E. aerogenes.
- Inhibition of K. pneumoniae growth was observed for 8 hours, with increased growth at 24 hours.
- Significant reduction and elimination of E. coli and P. aeruginosa were noted within 2 hours.
- A decrease in C. albicans growth was observed at 4 and 8 hours, with increased growth at 24 hours.
Conclusions:
- Peritoneal fluid exhibits a dose-dependent bactericidal effect against specific Gram-negative bacteria, notably E. coli and P. aeruginosa.
- The findings suggest that peritoneal fluid possesses innate antimicrobial properties that can combat key pathogens in intra-abdominal infections.
- Further research may elucidate the specific components within peritoneal fluid responsible for this observed bactericidal activity.
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