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Updated: Feb 3, 2026

Patterning Bioactive Proteins or Peptides on Hydrogel Using Photochemistry for Biological Applications
Published on: September 15, 2017
Light-Controlled Chemoenzymatic Immobilization of Proteins towards Engineering of Bioactive Papers
Valentina Hilberg1,2, Olga Avrutina1, Aileen Ebenig1
1Institut für Organische Chemie und Biochemie, Technische Universität Darmstadt, Alarich-Weiss-Strasse 4, 64287, Darmstadt, Germany.
Abstract:
Efficient and reliable methods for the generation of bioactive papers are of growing interest in relation to point-of-care testing devices that do not require extensive analytical equipment. Herein, we report the immobilization of functional proteins on paper fibers using a modular chemoenzymatic approach. The synthetic strategy relies on a combination of highly efficient spatially controllable photo-triggered cycloaddition followed by site-specific sortase A-catalyzed transamidation. This site-directed and regiospecific method has allowed unidirectional and covalent immobilization of several proteins displaying different functional properties, with ramifications for application in paper-based diagnostics.
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