Related Experiment Video

Updated: Feb 3, 2026

A High Yield and Cost-efficient Expression System of Human Granzymes in Mammalian Cells
09:16

A High Yield and Cost-efficient Expression System of Human Granzymes in Mammalian Cells

Published on: June 10, 2015

10.5K

Granzyme K Expressed by Classically Activated Macrophages Contributes to Inflammation and Impaired Remodeling

Christopher T Turner1, Matthew R Zeglinski1, Katlyn C Richardson1

  • 1International Collaboration On Repair Discoveries (ICORD) Centre, Vancouver Coastal Health Research Institute, University of British Columbia, Vancouver, British Columbia, Canada; Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, British Columbia, Canada.

The Journal of Investigative Dermatology
|November 6, 2018
PubMed

Insights

Granzyme K (GzmK) promotes inflammation and impairs skin healing after burns. Removing GzmK improved wound closure and tissue repair in mice, highlighting its role in thermal injury inflammation.

Area of Science:

  • Immunology
  • Dermatology
  • Protease research

Background:

  • Granzyme K (GzmK), a serine protease, is linked to inflammation and elevated in sepsis.
  • Its role in thermal injury and wound healing is not well understood.

Purpose of the Study:

  • To investigate the role of Granzyme K (GzmK) in inflammation and tissue remodeling following thermal injury.
  • To determine GzmK's impact on skin wound healing and epithelialization.

Main Methods:

  • Analyzing GzmK expression in human burn tissue and cultured macrophages.
  • Utilizing wild-type and GzmK knockout (GzmK-/-) mice subjected to thermal injury.
  • Assessing wound closure, matrix organization, tensile strength, and inflammatory markers (IL-6, ICAM-1, VCAM-1, MCP-1).
  • Investigating GzmK's effect on keratinocytes and fibroblasts in vitro.

Main Results:

  • GzmK was elevated in human burn tissue, primarily in macrophages.
  • GzmK knockout mice showed enhanced wound healing, improved matrix organization, and increased tensile strength.
  • Reduced levels of key inflammatory markers were observed in GzmK knockout mice.
  • GzmK induced IL-6 expression in keratinocytes and fibroblasts, dependent on PAR-1 activation.
  • GzmK impaired in vitro keratinocyte wound healing, suggesting sensitivity to its proteolysis.

Conclusions:

  • Granzyme K (GzmK) significantly contributes to inflammation and impedes epithelialization during thermal wound healing.
  • Targeting GzmK may offer a therapeutic strategy to improve skin wound repair.

More Related Videos

Isolation, Characterization, and Purification of Macrophages from Tissues Affected by Obesity-related Inflammation07:46

Isolation, Characterization, and Purification of Macrophages from Tissues Affected by Obesity-related Inflammation

Published on: April 3, 2017

26.0K
A Colorimetric Assay that Specifically Measures Granzyme B Proteolytic Activity: Hydrolysis of Boc-Ala-Ala-Asp-S-Bzl05:20

A Colorimetric Assay that Specifically Measures Granzyme B Proteolytic Activity: Hydrolysis of Boc-Ala-Ala-Asp-S-Bzl

Published on: November 28, 2014

13.8K

Related Concept Videos

Nucleosome Remodeling02:54

Nucleosome Remodeling

Nucleosomes are the basic units of chromatin compaction. Each nucleosome consists of the DNA bound tightly around a histone core, which makes the DNA inaccessible to DNA binding proteins such as DNA polymerase and RNA polymerase. Hence, the fundamental problem is to ensure access to DNA when appropriate, despite the compact and protective chromatin structure.
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...
11.2K
Inflammation01:38

Inflammation

Overview
62.1K
Classical Conditioning01:18

Classical Conditioning

Associative learning, a core principle in behavioral psychology, involves forming connections between events and facilitating learned responses. This concept is vividly illustrated by classical conditioning, a process extensively studied by the Russian physiologist Ivan Pavlov. Pavlov's pioneering research on dogs' digestive systems led to the discovery that behaviors can be learned through association, laying the groundwork for classical conditioning.
Ivan Pavlov observed that dogs...
2.2K
Binet's Contribution to Measures of Intelligence01:23

Binet's Contribution to Measures of Intelligence

Alfred Binet, along with his student Théophile Simon, was tasked by the French Ministry of Education in 1904 to create a method for identifying students who struggled to learn through conventional classroom instruction. This initiative aimed to address overcrowding by placing such students in specialized schools. Binet and Simon developed an intelligence test comprising 30 tasks, ranging from simple commands, like touching one's nose or ear, to more complex tasks, such as drawing...
1.7K
Bone Remodeling01:40

Bone Remodeling

Bone remodeling is a continuous and balanced process of bone resorption by osteoclasts and bone formation by osteoblasts. In adults, it helps maintain bone mass and calcium homeostasis. While mechanical stress can stimulate turnover as part of the normal maintenance and reparative process, several hormones also regulate bone remodeling.
40.4K
Principles of Classical Conditioning01:23

Principles of Classical Conditioning

Classical conditioning, as described by Ivan Pavlov, is a foundational concept in associative learning, where a neutral stimulus becomes capable of eliciting a conditioned response through association with an unconditioned stimulus. The process of acquisition, where this learning occurs, and the subsequent phenomena of contiguity, contingency, generalization, discrimination, extinction, and spontaneous recovery are crucial for a comprehensive understanding of classical conditioning.
During the...
1.9K