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Polymerase Chain Reaction: Basic Protocol Plus Troubleshooting and Optimization Strategies
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A study of polymerase chain reaction device control via cloud using Firebase Cloud Messaging protocol.

Jong-Dae Kim1,2, So-Yeon Lee1,2, Yu-Seop Kim1,2

  • 1Department of Convergence Software, Hallym University, 1, Hallymdaehak-gil, Chuncheon, Republic of Korea.

Biomedical Engineering Online
|November 7, 2018
PubMed
Summary
This summary is machine-generated.

Cloud-based control of Polymerase Chain Reaction (PCR) devices is not feasible due to slow data transmission. However, real-time external monitoring of PCR device status is achievable.

Keywords:
Cloud computingFirebase Cloud Messaging (FCM)Internet of Things (IoT)Polymerase chain reaction (PCR)Realtime monitoring

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Area of Science:

  • Biotechnology
  • Molecular Biology
  • Cloud Computing

Background:

  • Polymerase Chain Reaction (PCR) is a fundamental technique for DNA amplification.
  • Current PCR systems have limitations in external control and data access.
  • Existing systems are often standalone or require proximity to the PCR device.

Purpose of the Study:

  • To develop a cloud-based system for external data monitoring and control of PCR devices.
  • To overcome geographical limitations associated with traditional PCR system management.
  • To enhance user convenience through remote access to PCR experimental data and device operation.

Main Methods:

  • Utilized Google's Firebase cloud platform for system development.
  • Employed Firebase Cloud Messaging (FCM) protocol for data transmission between devices, cloud, and users.
  • Evaluated data transmission and reception feasibility for PCR devices requiring rapid temperature cycling (response time < 50 ms).

Main Results:

  • The average data round-trip time using FCM was measured at 150 ms.
  • FCM data exchange time was found to be three times slower than the required 50 ms.
  • This latency prevents real-time control of PCR devices requiring rapid temperature adjustments.

Conclusions:

  • Cloud-based real-time control of the studied PCR devices is not possible with the current FCM implementation.
  • The system enables real-time external monitoring of PCR device status.
  • Future work may explore alternative protocols for faster data exchange to enable full remote control.