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Related Experiment Videos

The maize transposable element Ds is spliced from RNA.

S R Wessler, G Baran, M Varagona

    Science (New York, N.Y.)
    |August 21, 1987
    PubMed
    Summary

    Maize transposable elements (Ds) can be spliced from RNA, allowing genes to retain function. This study shows how Ds elements are removed from messenger RNA, preserving wild-type gene transcripts.

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    Area of Science:

    • Molecular Biology
    • Genetics
    • Plant Science

    Background:

    • Maize transposable elements, like Dissociation (Ds), can insert into gene exons.
    • Gene expression can be maintained despite these insertions, suggesting RNA processing mechanisms.

    Purpose of the Study:

    • To investigate the RNA processing of maize Dissociation (Ds) elements inserted into exons.
    • To understand how wild-type size messenger RNAs are produced from genes containing Ds insertions.

    Main Methods:

    • Analysis of transcripts from maize waxy (wx) gene alleles with Ds insertions in exons.
    • Study conducted in strains where Ds element excision was prevented.
    • DNA sequencing of complementary DNAs to analyze splicing patterns.

    Main Results:

    • Predominant transcripts were wild-type size despite 4.3- and 1.5-kb Ds insertions.
    • Ds elements were spliced out of the messenger RNA using specific splice sites.
    • Multiple 5' donor splice sites within Ds termini and a 3' acceptor site in the wx gene were utilized.

    Conclusions:

    • Maize Ds elements can be effectively spliced from RNA transcripts.
    • This splicing mechanism allows for the production of functional, wild-type size messenger RNA.
    • Gene activity can be preserved even with transposable element insertions in coding regions.

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