Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Uncertainty in Measurement: Reading Instruments02:46

Uncertainty in Measurement: Reading Instruments

52.0K
Counting is the type of measurement that is free from uncertainty, provided the number of objects being counted does not change during the process. Such measurements result in exact numbers. By counting the eggs in a carton, for instance, one can determine exactly how many eggs are there in the carton. Similarly, the numbers of defined quantities are also exact. For example, 1 foot is exactly 12 inches, 1 inch is exactly 2.54 centimeters, and 1 gram is exactly 0.001 kilograms. Quantities...
52.0K
Protein Complex Assembly02:41

Protein Complex Assembly

16.8K
Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
16.8K
Protein Complex Assembly02:41

Protein Complex Assembly

2.6K
2.6K
Spindle Assembly02:50

Spindle Assembly

4.3K
Spindle assembly occurs through three, often coexisting, pathways – the centrosome-mediated pathway, the chromatin-mediated pathway, and the microtubule-mediated pathway – collectively contributing to form a robust spindle apparatus.
In most cells, centrosomes are the primary microtubule nucleation centers. In the centrosome-mediated pathway, the G2-prophase transition triggers centrosome maturation and increased microtubule nucleation. Progressive nucleation results in a...
4.3K
Oligosaccharide Assembly01:24

Oligosaccharide Assembly

3.7K
Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
Multiple sugar molecules that may or may...
3.7K
Assembly of Cytoskeletal Filaments01:18

Assembly of Cytoskeletal Filaments

27.9K
Cytoskeletal filaments are polymeric forms of smaller protein subunits. However, individual cytoskeletal filaments may easily disassemble or associate with other similar filaments to form rigid structures. Microfilaments, made of actin monomers, rely on actin-binding proteins to form bundles and create networks of individual actin filaments. Microtubules rely on microtubule-associated proteins (MAPs) to form sturdy cylindrical structures. However, the proteins involved in forming complex...
27.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Unique <i>SLC26A4</i> Mutation Spectrum in a Mongolian Enlarged Vestibular Aqueduct Cohort via Whole-Exome Sequencing: A Preliminary Study.

International journal of molecular sciences·2026
Same author

Genotype and subependymal lesion burden influence volumetric response to everolimus in tuberous sclerosis complex-associated subependymal giant cell astrocytoma: A 10-year real-world study.

Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics·2026
Same author

Lipidomic profiling of plasma triglyceride species reveals distinct signatures and dietary associations in drug-refractory hypertriglyceridemia.

Clinical nutrition (Edinburgh, Scotland)·2026
Same author

Surgeon educators are infrastructure: Governing surgical training at scale.

American journal of surgery·2026
Same author

Neonatal exchange blood is a rich source of hematopoietic stem and progenitor cells for transplantation.

Haematologica·2026
Same author

ABCA4 Versus PRPH2-Associated Retinopathy: Clinical and Electrophysiological Findings.

Investigative ophthalmology & visual science·2026
Same journal

Assessing the sustainability of reef and demersal fish stocks in Northwest México under a data-limited approach.

PeerJ·2026
Same journal

The impact of virtual reality exercise programs on postpartum pelvic pain and disability among women with lumbopelvic pain.

PeerJ·2026
Same journal

Soil salinity modulates fatty acid composition and antioxidant capacity of rice bran oil.

PeerJ·2026
Same journal

The optimal dose of brisk walking for improving blood pressure in hypertensive patients: a systematic review and bayesian meta-analysis of randomized controlled trials.

PeerJ·2026
Same journal

Enhanced sweet pepper yield through high-intensity artificial lighting and optimized plant density in high-latitude winter production.

PeerJ·2026
Same journal

Prenatal corticosteroid use improves the severity and complications of necrotizing enterocolitis in preterm infants: a retrospective multicenter clinical study in China.

PeerJ·2026
See all related articles

Related Experiment Video

Updated: Feb 3, 2026

Heuristic Mining of Hierarchical Genotypes and Accessory Genome Loci in Bacterial Populations
08:03

Heuristic Mining of Hierarchical Genotypes and Accessory Genome Loci in Bacterial Populations

Published on: December 7, 2021

2.8K

HAHap: a read-based haplotyping method using hierarchical assembly.

Yu-Yu Lin1, Ping Chun Wu2, Pei-Lung Chen3

  • 1Department of Graduate Institute of Biomedical Electronics and Bioinformatics, National Taiwan University, Taipei, Taiwan.

Peerj
|November 7, 2018
PubMed
Summary
This summary is machine-generated.

This study introduces HAHap, a novel hierarchical assembly algorithm for haplotype phasing. HAHap improves accuracy by progressively resolving local conflicts, outperforming existing minimum error correction methods.

Keywords:
HaplotypeHierarchical assemblyProbability scoreRead-based phasing

More Related Videos

Versatile Technique to Produce a Hierarchical Design in Nanoporous Gold
05:28

Versatile Technique to Produce a Hierarchical Design in Nanoporous Gold

Published on: February 10, 2023

2.2K
Hierarchical and Programmable One-Pot Oligosaccharide Synthesis
09:56

Hierarchical and Programmable One-Pot Oligosaccharide Synthesis

Published on: September 6, 2019

7.3K

Related Experiment Videos

Last Updated: Feb 3, 2026

Heuristic Mining of Hierarchical Genotypes and Accessory Genome Loci in Bacterial Populations
08:03

Heuristic Mining of Hierarchical Genotypes and Accessory Genome Loci in Bacterial Populations

Published on: December 7, 2021

2.8K
Versatile Technique to Produce a Hierarchical Design in Nanoporous Gold
05:28

Versatile Technique to Produce a Hierarchical Design in Nanoporous Gold

Published on: February 10, 2023

2.2K
Hierarchical and Programmable One-Pot Oligosaccharide Synthesis
09:56

Hierarchical and Programmable One-Pot Oligosaccharide Synthesis

Published on: September 6, 2019

7.3K

Area of Science:

  • Genomics
  • Bioinformatics

Background:

  • Advances in sequencing necessitate read-based phasing for haplotype resolution.
  • Minimum Error Correction (MEC) is a common approach but can be misled by noisy data.
  • Existing MEC methods may select suboptimal haplotypes due to global conflict resolution.

Purpose of the Study:

  • To present HAHap, a hierarchical assembly-based algorithm for accurate haplotype phasing.
  • To address limitations of current MEC methods in handling noisy sequencing data.

Main Methods:

  • Developed HAHap, a novel algorithm utilizing hierarchical assembly.
  • Employed high-confident variant pairs for progressive haplotype construction.
  • Evaluated HAHap on both real and simulated whole-genome sequencing data.

Main Results:

  • HAHap demonstrated superior phasing accuracy compared to MEC-based methods.
  • The algorithm achieved lower error correction rates on real datasets.
  • Simulated data analysis confirmed HAHap's effectiveness under various sequencing conditions.

Conclusions:

  • HAHap offers a more accurate approach to read-based phasing, especially with short reads.
  • The hierarchical assembly strategy effectively resolves local conflicts missed by global MEC methods.
  • HAHap shows promise for improving haplotype resolution in genomic studies.