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Related Experiment Video

Updated: Feb 3, 2026

Protein Engineering by Yeast Surface Display

Protein Engineering by Yeast Surface Display

Published on: November 29, 2024

Engineering of Yeast Glycoprotein Expression.

Charlot De Wachter^1,2, Linde Van Landuyt^1,2, Nico Callewaert^3,4

¹VIB-UGent Center for Medical Biotechnology, Ghent, Belgium.

Advances in Biochemical Engineering/Biotechnology

|November 7, 2018

Summary

Glyco-engineering yeast hosts improves recombinant protein production by modifying glycosylation. This overcomes immune responses and rapid clearance, making yeast suitable for biopharmaceutical manufacturing.

Keywords:

Fungi N-glycosylation engineering O-glycosylation engineering Pichia pastoris Recombinant protein expression Saccharomyces cerevisiae Yeast

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Expression of Recombinant Proteins in the Methylotrophic Yeast Pichia pastoris

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Published on: February 25, 2010

Area of Science:

Biotechnology
Molecular Biology
Biopharmaceutical Manufacturing

Background:

Yeasts are efficient hosts for recombinant protein production due to rapid growth and cost-effective media.
A significant limitation is yeast-specific glycosylation, which differs from mammalian cells and can cause adverse effects on therapeutic proteins.
Glycosylation impacts protein folding, pharmacokinetics, and pharmacodynamics, and yeast's hypermannosylated structures can trigger immune responses.

Purpose of the Study:

To address the challenge of yeast-specific glycosylation in recombinant protein production.
To highlight advancements in glyco-engineering strategies for yeast hosts.
To enable yeast to regain its position in biopharmaceutical manufacturing.

Main Methods:

Glyco-engineering of N- and O-type glycosylation pathways in yeast.
Removal or reduction of yeast-specific glycans.
Introduction of humanized glycosylation pathways.

Main Results:

Development of effective solutions for modifying yeast glycosylation.
Overcoming challenges associated with yeast-specific glycan structures.
Enabling the production of therapeutic proteins with improved properties.

Conclusions:

Glyco-engineered yeasts offer a viable solution for producing safe and effective biopharmaceuticals.
These advancements are crucial for optimizing recombinant protein production in yeast.
Yeast hosts are poised to become leading platforms for biopharmaceutical manufacturing once again.