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Towards metrologically traceable and comparable results in GM quantification.

Philippe Corbisier1, Hendrik Emons2

  • 1European Commission, Joint Research Centre (JRC), Retieseweg 111, 2440, Geel, Belgium.

Analytical and Bioanalytical Chemistry
|November 7, 2018
PubMed
Summary
This summary is machine-generated.

This study introduces a reference measurement system for genetically modified (GM) content in food and feed. It ensures comparable results for regulatory decision-making by establishing metrological traceability for GM mass fraction quantification.

Keywords:
Conversion factorGenetically modified organismPCRQuantificationTraceabilityUnit of measurement

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Area of Science:

  • Food science and technology
  • Analytical chemistry
  • Biotechnology

Background:

  • European regulations require GM content in food/feed to be expressed as a GM mass fraction.
  • Current PCR-based quantification methods primarily count DNA fragments, posing challenges for accurate mass fraction determination.

Purpose of the Study:

  • To outline requirements for obtaining comparable GM quantification results.
  • To introduce a reference measurement system for metrological traceability in GMO analysis.
  • To ensure measurement results are suitable for regulatory decision-making.

Main Methods:

  • Development of a reference measurement system for GMO analysis.
  • Establishing metrological traceability to a unique reference point.
  • Defining conversion factors with minimum uncertainty, anchored to certified reference materials.

Main Results:

  • The proposed system enables GMO analysis laboratories to relate their results to a universally accessible reference.
  • It facilitates the transformation of data between measurement units with specified uncertainty.
  • Calibration approaches for achieving comparable GM quantification results are outlined.

Conclusions:

  • A robust reference measurement system is crucial for accurate and comparable GM content quantification.
  • Metrological traceability and certified reference materials are key to reliable regulatory compliance.
  • The outlined methods support consistent and legally sound GM analysis in food and feed products.