Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Mapping structurally perturbed sites in DNA by replication arrest and run-off replication.

Y Wang, W Sauerbier

    Biochemical and Biophysical Research Communications
    |August 14, 1987
    PubMed
    Summary

    This study introduces a faster DNA mapping technique to pinpoint structural changes. The method uses DNA sequencing reactions and gel electrophoresis for precise identification of torsion-induced DNA perturbations.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Genomic studies of the Lucké tumor herpesvirus (RaHV-1).

    Journal of cancer research and clinical oncology·1999
    Same author

    Structure and transcription of the gene for translation elongation factor 1 subunit alpha of zebrafish (Danio rerio).

    Biochimica et biophysica acta·1997
    Same author

    Zebrafish translation elongation factor EF1 alpha mRNA: sequence and secondary structures.

    Molecular marine biology and biotechnology·1996
    Same author

    Fate of herpesvirus DNA in embryos and tadpoles cloned from Lucké renal carcinoma nuclei.

    Journal of comparative pathology·1994
    Same author

    Parasexual crosses and hybrid selection with a near haploid variant of the epithelioma papulosum cyprini, cell line EPC: a tissue culture model for the analyses of dominance, recessiveness, and complementation of mutant phenotypes.

    Molecular marine biology and biotechnology·1994
    Same author

    The presence of DNA sequences of the Lucké herpesvirus in normal and neoplastic kidney tissue of Rana pipiens.

    Journal of comparative pathology·1994

    Area of Science:

    • Molecular Biology
    • Genomics
    • Biochemistry

    Background:

    • DNA structure is dynamic and can be perturbed by torsional stress.
    • Identifying these structural perturbations is crucial for understanding DNA replication and repair.
    • Current methods for mapping DNA structural changes are often complex and time-consuming.

    Purpose of the Study:

    • To develop a rapid and efficient technique for fine mapping DNA structural perturbations.
    • To improve upon existing methods for identifying sites of torsion-induced DNA alterations.

    Main Methods:

    • The technique involves strand scission or chemical modification at structurally perturbed DNA sites.
    • Replication is arrested within a double-strand DNA sequencing reaction.
    • Replication products are analyzed by size using electrophoresis on sequencing gels.

    Related Experiment Videos

    Main Results:

    • The described technique allows for rapid fine mapping of DNA structural perturbations.
    • It is less complicated and faster than conventional end-labeling methods.
    • High sequence specificity is achieved using oligomeric sequencing primers.

    Conclusions:

    • This novel technique offers a faster and more specific approach to mapping DNA structural changes.
    • The method is suitable for in vivo mapping of DNA structural perturbations within complex genomes.
    • It provides a valuable tool for studying DNA dynamics and genome stability.