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Updated: Feb 3, 2026

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
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Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

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Werner Syndrome Protein and DNA Replication.

Shibani Mukherjee1, Debapriya Sinha2, Souparno Bhattacharya3

  • 1Division of Molecular Radiation Biology, Department of Radiation Oncology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA. Shibani.Mukherjee@utsouthwestern.edu.

International Journal of Molecular Sciences
|November 8, 2018
PubMed
Summary

Werner Syndrome Protein (WRN) is vital for maintaining genome stability and preventing premature aging. This review highlights WRN

Keywords:
DNA double-strand repairWerner SyndromeWerner Syndrome Proteincancerpost-translational modificationpremature agingprotein stabilityreplication stress

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Area of Science:

  • Genetics and Molecular Biology
  • Cellular Biology
  • Biochemistry

Background:

  • Werner Syndrome (WS) is a premature aging disorder linked to mutations in the Werner Syndrome Protein (WRN).
  • WRN protein is crucial for DNA replication, repair, and recombination, and its dysfunction leads to genomic instability.
  • Individuals with WS exhibit increased susceptibility to rare mesenchymal cancers.

Purpose of the Study:

  • To review the role of human WRN in replication fork processing.
  • To elucidate WRN's contribution to maintaining genome stability and suppressing premature aging.
  • To understand WRN's molecular functions in DNA replication and their implications for WS pathophysiology.

Main Methods:

  • Literature review focusing on human WRN.
  • Analysis of WRN's interactions with DNA replication, repair, and recombination factors.
  • Examination of WRN's post-translational modifications, including phosphorylation by various kinases.

Main Results:

  • WRN possesses unique exonuclease and helicase activities essential for DNA metabolism.
  • WRN forms dynamic complexes with factors involved in DNA replication, recombination, and repair.
  • Phosphorylation of WRN by kinases like ATM and ATR is critical for its function in response to genotoxic stress.

Conclusions:

  • WRN plays a critical role in genome stability maintenance pathways, preventing premature aging and cancer.
  • WRN facilitates replication fork progression and the repair of stalled forks and associated DNA double-strand breaks.
  • Understanding WRN's function in DNA replication is key to unraveling Werner Syndrome.