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Updated: Feb 3, 2026

Targeted DNA Methylation Analysis by Next-generation Sequencing
Published on: February 24, 2015
DNA methylation and de-methylation using hybrid site-targeting proteins
Yong Lei1,2, Yung-Hsin Huang1,2,3, Margaret A Goodell4,5,6,7
1Stem Cells and Regenerative Medicine Center, Baylor College of Medicine, Houston, TX, 77030, USA.
Abstract:
DNA methylation plays important roles in determining cellular identity, disease, and environmental responses, but little is known about the mechanisms that drive methylation changes during cellular differentiation and tumorigenesis. Meanwhile, the causal relationship between DNA methylation and transcription remains incompletely understood. Recently developed targeted DNA methylation manipulation tools can address these gaps in knowledge, leading to new insights into how methylation governs gene expression. Here, we summarize technological developments in the DNA methylation editing field and discuss the remaining challenges facing current tools, as well as potential future directions.
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