Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Active immunization using hand-push emulsification method increases the operator's risk of transcutaneous

Xiaochen Yin1, Suli Zhang2, Zhen Zhou3

  • 1Department of Physiology & Pathophysiology, School of Basic Medical Sciences, Capital Medical University, Beijing, China.

Biochemical and Biophysical Research Communications
|November 8, 2018
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Feasibility and optimal imaging time window for intraperitoneal versus intravenous injection of a macrocyclic gadolinium-based contrast agent in mice.

The Journal of international medical research·2026
Same author

Spatial transcriptomics reveals coordinated ventricular patterning and maturation in the developing human heart.

Nature communications·2026
Same author

Empathy fatigue in ecosystem conservation: The awareness decline among concerns over Japan's nuclear wastewater discharge.

Marine pollution bulletin·2026
Same author

β-Adrenergic receptors as immunomodulators in T cells: mechanisms of neuroimmune crosstalk and pathophysiological implications.

Molecular medicine (Cambridge, Mass.)·2026
Same author

Progress in the Treatment of Ulcerative Colitis by Targeting NLRP3 Inflammasome.

Drug design, development and therapy·2026
Same author

Mesenchymal neoplasms with RAF/BRAF alterations: eight cases revealing novel fusions, V600E mutation and clonal evolution.

Journal of clinical pathology·2026
This summary is machine-generated.

Researchers accidentally exposed to emulsified antigens during animal model setup developed high levels of angiotensin II type 1 receptor autoantibodies (AT1-AA). Using automatic emulsification devices significantly reduced AT1-AA levels, preventing researcher infection.

Area of Science:

  • Immunology
  • Occupational Health

Background:

  • Active immunization is crucial for studying immune diseases and antibody production.
  • The safety of pathogen emulsification during animal model setup is not well understood.
  • Unexpectedly high levels of angiotensin II type 1 receptor autoantibody (AT1-AA) were observed in researchers involved in animal model creation.

Purpose of the Study:

  • To investigate if emulsified antigens caused AT1-AA production in researchers.
  • To identify the transmission route of emulsified antigens.
  • To develop preventive measures against researcher infection during immunization.

Main Methods:

  • Detection of AT1-AA using advanced ELISA.
  • Comparison of AT1-AA levels between researchers and non-participants.
Keywords:
Active immunization modelAutoantibodiesEmulsified antigenExperimental security

Related Experiment Videos

  • Animal and glove penetration studies to assess infection routes.
  • Evaluation of AT1-AA levels after using an automatic emulsification device.

    • Main Results:

    • Researchers involved in active immunization showed significantly higher AT1-AA levels than controls.
    • Infection was not attributed to aerosols, fur contact, or glove penetration.
    • AT1-AA levels decreased substantially when an automatic emulsification device was employed.
    • Accidental skin contact with emulsified antigens, facilitated by adjuvant permeability, was identified as the likely transmission route.

    Conclusions:

    • Traditional hand-push emulsification poses an infection risk to researchers through accidental contact with emulsified antigens.
    • This exposure can lead to elevated autoantibody levels, specifically AT1-AA.
    • Employing automatic emulsification devices is an effective preventive strategy to mitigate researcher infection and autoantibody production.