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Related Concept Videos

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Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
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Updated: Feb 3, 2026

Chromatin Interaction Analysis with Paired-End Tag Sequencing ChIA-PET for Mapping Chromatin Interactions and Understanding Transcription Regulation
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Noncoding transcription influences the replication initiation program through chromatin regulation.

Julien Soudet1, Jatinder Kaur Gill1, Françoise Stutz1

  • 1Department of Cell Biology, University of Geneva, 1211 Genève 4, Switzerland.

Genome Research
|November 8, 2018
PubMed
Summary

Genomic noncoding transcription near autonomously replicating sequences (ARSs) in yeast regulates DNA replication initiation. Transcription termination shields ARS activity, influencing replication timing via chromatin modifications like H3K36 methylation.

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Area of Science:

  • Molecular Biology
  • Genetics
  • Epigenetics

Background:

  • Replication initiation in eukaryotes follows a temporal program, influenced by chromatin structure.
  • The role of genomic noncoding transcription in regulating replication initiation has been largely overlooked.

Purpose of the Study:

  • To investigate the impact of noncoding transcription on replication initiation in *Saccharomyces cerevisiae*.
  • To elucidate the mechanisms by which transcription influences replication timing and ARS efficiency.

Main Methods:

  • Analysis of natural nascent transcription in relation to ARS efficiency and replication timing.
  • Investigating the effects of forced ARS readthrough transcription on chromatin features.
  • Assessing replication initiation defects and rescue in yeast mutants with altered H3K36 methylation.

Main Results:

  • Noncoding RNA transcription termination near ARSs protects replication initiation from transcriptional readthrough.
  • High nascent transcription correlates with reduced ARS efficiency and later replication timing.
  • Increased readthrough transcription is associated with higher nucleosome occupancy and H3K36me3 levels.
  • Replication initiation defects caused by readthrough transcription are partially rescued by removing H3K36 methylation.

Conclusions:

  • Genomic noncoding transcription influences replication initiation in yeast.
  • This regulation occurs through transcription-mediated chromatin modifications, particularly H3K36 methylation.
  • Transcription termination near ARSs is crucial for efficient and timely replication initiation.