Related Experiment Video
Updated: Feb 2, 2026

Two Methods of Heterokaryon Formation to Discover HCV Restriction Factors
Published on: July 16, 2012
Using restricted factor analysis to select anchor items and detect differential item functioning
Laura Kolbe1, Terrence D Jorgensen2
1Department of Child Development and Education, University of Amsterdam, Amsterdam, The Netherlands. L.Kolbe@uva.nl.
Abstract:
Restricted factor analysis (RFA) is a powerful method to test for uniform differential item functioning (DIF), but it may require empirically selecting anchor items to prevent inflated Type I error rates. We conducted a simulation study to compare two empirical anchor-selection strategies: a one-step rank-based strategy and an iterative selection procedure. Unlike the iterative procedure, the rank-based strategy had a low risk and degree of contamination within the empirically selected anchor set, even with small samples. To detect nonuniform DIF, RFA requires an interaction effect with the latent factor. The latent moderated structural equations (LMS) method has been applied to RFA and has revealed inflated Type I error rates. We propose using product indicators (PI) as a more widely available alternative to measure the latent interaction. A simulation study, involving several sample-size conditions and magnitudes of uniform and nonuniform DIF, revealed that PI obtained similar power but lower Type I error rates, as compared to LMS.
More Related Videos
09:58Mapping the Structure-Function Relationships of Disordered Oncogenic Transcription Factors Using Transcriptomic Analysis
Published on: June 27, 2020
12:44Identification of Key Factors Regulating Self-renewal and Differentiation in EML Hematopoietic Precursor Cells by RNA-sequencing Analysis
Published on: November 11, 2014
Related Concept Videos
The Anchoring-and-Adjustment Heuristic
Anchoring Junctions
Lipids as Anchors
The carboxy-terminal of most of the prenylated proteins, such as Ras proteins, contains...
Transcription Factors
Restriction Enzymes
The host bacteria protect their own genomic DNA from these enzymes by methylating these sites. Some...
GPI Anchoring of Proteins in the ER Membrane
GPI-anchor structure
A sequence of 11 enzymatic reactions results in the synthesis of the complete GPI anchor consisting of a hydrophobic and a hydrophilic portion. The hydrophobic portion comprises phosphatidylinositol, while the hydrophilic part comprises polar groups like phosphoethanolamine,...