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Related Concept Videos

Atomic Force Microscopy01:08

Atomic Force Microscopy

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Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
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Cell Adhesion Molecules - Types and Functions01:20

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Cell adhesion molecules (CAMs) are pivotal to multicellularity and the coordinated functioning of tissues and organ systems. They enable physical interactions between cells and provide mechanical strength to tissues. They also function as receptors for signal transmission across the plasma membrane. The CAMs are broadly classified into four families - integrins, cadherins, selectins, and immunoglobulin-like CAMs (IgCAMs).
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Adhesion01:14

Adhesion

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Adhesion occurs when one type of molecule is attracted to a different molecule. Water exhibits adhesive properties in the presence of polar surfaces, such as glass or cellulose in plants. For instance, when water is poured into a glass, the positively charged hydrogen molecules of water are more attracted to the negatively charged oxygen molecules in the silica than to the oxygen in neighboring water molecules.
Capillary action is a result of water’s adhesive tendencies. When a narrow...
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Atomic Structure01:33

Atomic Structure

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Overview
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Intermolecular Forces and Physical Properties02:56

Intermolecular Forces and Physical Properties

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Functional Groups02:45

Functional Groups

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Functional groups are a group of atoms with characteristic properties, which when linked to the carbon skeleton of a molecule, alter the properties of that molecule. For example, the presence of certain functional groups on a molecule will make them hydrophilic, whereas others will make them hydrophobic. These functional groups are an indispensable part of organic chemistry and important components of biological molecules, such as carbohydrates, proteins, lipids, and nucleic acids. Each...
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Related Experiment Video

Updated: Feb 2, 2026

Characterizing Mechanical Properties of Primary Cell Wall in Living Plant Organs Using Atomic Force Microscopy
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Characterizing Mechanical Properties of Primary Cell Wall in Living Plant Organs Using Atomic Force Microscopy

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Quantitative characterization of single-cell adhesion properties by atomic force microscopy using

Lionel Chièze1, Anthony Le Cigne1, Marie Meunier2

  • 1Laboratoire de Recherche en Nanosciences, LRN EA4682, Université de Reims Champagne-Ardenne, Reims, France.

Journal of Molecular Recognition : JMR
|November 8, 2018
PubMed
Summary

This study introduces an atomic force microscopy (AFM) method to precisely measure single cell adhesion. This technique reveals individual cell differences missed by bulk assays, aiding research in areas like cancer cell migration.

Keywords:
atomic force microscopy (AFM)cancercell adhesioncell migrationforce spectroscopytip functionalization

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Measuring the Mechanical Properties of Living Cells Using Atomic Force Microscopy
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Measuring the Mechanical Properties of Living Cells Using Atomic Force Microscopy
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Area of Science:

  • Biophysics
  • Cell Biology
  • Materials Science

Background:

  • Traditional bulk assays offer limited resolution for cell adhesion properties.
  • Existing methods provide semiquantitative, averaged data, masking individual cell variations.
  • Understanding single-cell adhesion is crucial for fields like cancer research.

Purpose of the Study:

  • To develop and validate a novel atomic force microscopy (AFM) method for characterizing single-cell adhesion.
  • To demonstrate the quantitative and reproducible assessment of cell-substrate interactions.
  • To highlight the limitations of bulk techniques compared to single-cell force spectroscopy.

Main Methods:

  • Utilizing protein-functionalized atomic force microscopy (AFM) probes for cell adhesion analysis.
  • Employing force spectroscopy to quantify the mean detachment force between single cells and a functionalized colloidal tip.
  • Comparing AFM-based measurements with traditional washing assays.

Main Results:

  • The AFM method quantitatively differentiates cell adhesion properties at the single-cell level.
  • This technique identified variations in adhesion not detectable by bulk washing assays.
  • AFM enabled reproducible measurements under standard cell culture conditions.
  • The method successfully detected distinct subpopulations of cells based on adhesion strength.

Conclusions:

  • AFM-based force spectroscopy provides a superior method for analyzing single-cell adhesion.
  • This technique overcomes the limitations of bulk assays, offering higher resolution and sensitivity.
  • The developed method is valuable for investigating cellular heterogeneity and its implications in various biological processes, including cancer cell migration.