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Thermal Measurement Techniques in Analytical Microfluidic Devices
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High-Resolution Microfluidic Paper-Based Analytical Devices for Sub-Microliter Sample Analysis.

Keisuke Tenda1, Riki Ota2, Kentaro Yamada3

  • 1Department of Applied Chemistry, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522, Kanagawa, Japan. kei-54ppp@keio.jp.

Micromachines
|November 9, 2018
PubMed
Summary

Researchers developed novel microfluidic paper-based analytical devices (µPADs) for analyzing tiny liquid samples. This advancement uses a simpler hot lamination technique for precise microfluidic channel fabrication, enabling sensitive protein analysis with minimal sample volumes.

Keywords:
colorimetryinkjet printingprotein assaywax printingµPAD

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Area of Science:

  • Microfluidics
  • Analytical Chemistry
  • Materials Science

Background:

  • Microfluidic paper-based analytical devices (µPADs) are cost-effective platforms for point-of-care diagnostics.
  • Traditional fabrication methods for µPADs can be complex and require specialized equipment.
  • Analyzing sub-microliter sample volumes presents challenges in achieving reliable and reproducible results.

Purpose of the Study:

  • To demonstrate the fabrication of high-resolution microfluidic structures on paper substrates using an adapted wax-printing approach.
  • To develop a model µPAD design capable of analyzing very small sample volumes (sub-microliter).
  • To validate the performance of the fabricated µPADs using a colorimetric assay for total protein concentration.

Main Methods:

  • Adapted wax-printing technique using hot lamination instead of hot plate heating for patterning filter paper.
  • Optimization of device geometry and consideration of cellulose fiber direction for microfluidic channel design.
  • Development of a four-channel µPAD model with a minimum liquid filling volume of 0.5 µL.
  • Application of a colorimetric assay for quantifying total protein concentrations using human serum albumin (HSA).

Main Results:

  • Achieved high-resolution microfluidic structures on filter paper via hot lamination-based wax printing.
  • Developed a µPAD design capable of precise liquid handling as low as 0.5 µL.
  • Successfully performed a colorimetric assay for total protein quantification using sub-microliter samples (0.8 µL).
  • Demonstrated robustness with tolerance to ±0.1 µL variations in applied liquid volume during HSA calibration.

Conclusions:

  • Hot lamination offers a simplified and effective method for fabricating high-resolution µPADs.
  • The developed µPADs are suitable for the precise analysis of sub-microliter sample volumes.
  • This technology holds promise for sensitive and reliable biochemical assays requiring minimal sample input.