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Related Experiment Video

Updated: Feb 2, 2026

De Novo Generation of Somatic Stem Cells by YAP/TAZ

De Novo Generation of Somatic Stem Cells by YAP/TAZ

Published on: May 7, 2018

Enigma proteins regulate YAP mechanotransduction.

Ahmed Elbediwy¹, Hannah Vanyai², Maria-Del-Carmen Diaz-de-la-Loza²

¹Epithelial Biology Laboratory, The Francis Crick Institute, 1 Midland Rd, London NW1 1AT, UK barry.thompson@crick.ac.uk ahmed.elbediwy@crick.ac.uk.

Journal of Cell Science

|November 9, 2018

Summary

This summary is machine-generated.

Cellular forces activate YAP by recruiting Enigma proteins to integrin adhesions. These PDLIM proteins bind YAP, promoting its nuclear entry and gene activation during mechanotransduction.

Keywords:

Enigma Hippo Integrin Mechanotransduction PDLIM5 PDLIM7 YAP

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Area of Science:

Cell Biology
Mechanobiology
Molecular Biology

Background:

Human cells sense mechanical stress via integrin adhesions.
This stress response involves YAP (Yes-associated protein) and TAZ transcriptional co-activators.
The precise mechanism of integrin signaling activating YAP is not fully understood.

Purpose of the Study:

To elucidate the role of Enigma and Enigma-like proteins (PDLIM5/7) in integrin-mediated mechanotransduction.
To investigate how these proteins regulate YAP nuclear localization and activity.
To determine the mechanism by which forces trigger YAP activation.

Main Methods:

Silencing of PDLIM5/7 expression.
YAP binding assays using PDLIM5/7 C-terminal PDZ-binding motif (PBM).
Recruitment of PDLIM5/7 to integrin adhesions and F-actin stress fibers in response to force.
Binding of PDLIM5/7 to α-actinin.

Main Results:

Integrin-mediated mechanotransduction requires PDLIM5/7 proteins.
YAP binds to PDLIM5/7 via its PBM, which is crucial for YAP's nuclear localization and activity.
Silencing PDLIM5/7 reduces YAP nuclear localization, tyrosine phosphorylation, and transcriptional activity.
PDLIM5/7 proteins are recruited to integrin adhesions and stress fibers by binding to α-actinin under force.

Conclusions:

PDLIM5/7 proteins are essential mediators of integrin-driven mechanotransduction.
Force-induced recruitment of PDLIM5/7 to integrin adhesions triggers YAP activation.
This mechanism highlights a novel pathway for regulating YAP activity in response to mechanical stress.