Related Experiment Video
Updated: Jul 28, 2026
07:02
Identifying Dysregulated Genes Induced by Kaposi's Sarcoma-associated Herpesvirus (KSHV)
Published on: September 14, 2010
Genotypic analysis of large cell lymphomas which express the Ki-1 antigen
Histopathology
|July 1, 1987
Summary
The monoclonal antibody Ki-1 is expressed in certain non-Hodgkin's lymphomas. DNA analysis reveals these lymphomas can originate from T-cells, B-cells, or an unknown lineage, confirming diverse cellular origins.
Area of Science:
- Hematology
- Oncology
- Immunology
Background:
- The Ki-1 antibody identifies Reed-Sternberg cells in Hodgkin's disease.
- It also reacts with tumor cells in a subset of large cell non-Hodgkin's lymphomas.
- Understanding the cellular origin of Ki-1 positive lymphomas is crucial for diagnosis and treatment.
Purpose of the Study:
- To investigate the cellular lineage of non-Hodgkin's lymphomas expressing the Ki-1 antigen.
- To correlate immunophenotypic findings with genotypic analysis.
Main Methods:
- Immunophenotypic analysis of 30 non-Hodgkin's lymphoma cases.
- DNA analysis using immunoglobulin and T-cell receptor gene probes.
- Genotypic assessment to determine cellular origin.
Main Results:
- Sixteen cases demonstrated a monoclonal T-cell origin.
- Six cases were of monoclonal B-cell origin.
- Eight cases showed no evidence of T- or B-cell lineage.
Conclusions:
- Non-Hodgkin's lymphomas expressing the Ki-1 antigen can arise from either T-cell or B-cell lineages.
- A significant proportion of Ki-1 positive lymphomas may have an indeterminate lineage based on current genotypic analysis.
- These findings support the heterogeneity of Ki-1 positive lymphomas.
