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Related Concept Videos

Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

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Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
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Induced Pluripotent Stem Cells01:06

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Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
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Embryonic Stem Cells00:58

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Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
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Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
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Adult Stem Cells01:33

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Stem cells are undifferentiated cells that divide and produce more stem cells or progenitor cells that differentiate into mature, specialized cell types. All the cells in the body are generated from stem cells in the early embryo, but small populations of stem cells are also present in many adult tissues including the bone marrow, brain, skin, and gut. These adult stem cells typically produce the various cell types found in that tissue—to replace cells that are damaged or to continuously...
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A stem cell is an unspecialized cell that can divide without limit as needed and can, under specific conditions, differentiate into specialized cells.
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Transfecting and Nucleofecting Human Induced Pluripotent Stem Cells
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A Human Gonadal Cell Model From Induced Pluripotent Stem Cells.

Daniel Rodríguez Gutiérrez1, Wassim Eid1,2, Anna Biason-Lauber1

  • 1Section of Medicine, Endocrinology Division, University of Fribourg, Fribourg, Switzerland.

Frontiers in Genetics
|November 9, 2018
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Summary

Researchers developed a new model of Sertoli-like cells (SLCs) from human fibroblasts. These patient-specific cells show promise for studying male gonad development and treating disorders of sex development (DSD).

Keywords:
DSDNGSSertoli cellscell modeldisorders/differences of sex developmentiPSCreprograming

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Area of Science:

  • Reproductive biology
  • Stem cell research
  • Developmental biology

Background:

  • Sertoli cells are crucial for male gonad development and understanding 46,XY disorders of sex development (DSD).
  • Existing Sertoli cell models have limitations, often derived from cancer tissues or lacking proliferative capacity in vitro.
  • Mature primary Sertoli cells do not proliferate well in long-term cultures.

Purpose of the Study:

  • To generate and characterize a novel Sertoli-like cell (SLC) model derived from human fibroblasts (HFs).
  • To assess the potential of induced pluripotent stem cells (iPSCs) for creating patient-specific Sertoli cells.
  • To evaluate the utility of this new model for basic research and personalized medicine in reproductive health.

Main Methods:

  • Differentiated human fibroblast-derived induced pluripotent stem cells (iPSCs) into Sertoli-like cells (SLCs).
  • Utilized Next-Generation Sequencing (NGS) for comprehensive gene expression analysis.
  • Analyzed transcriptional profiles to assess cell maturation and marker expression.

Main Results:

  • The generated SLCs exhibited reduced pluripotency marker expression.
  • SLCs expressed key Sertoli cell markers, including SOX9, VIM, and CLDN-11.
  • Transcriptional profiling indicated an early stage of Sertoli cell maturation, with genetic and functional similarities to human Sertoli cells (HSerCs).

Conclusions:

  • A new model of Sertoli-like cells (SLCs) was successfully generated from human fibroblasts.
  • These SLCs demonstrate characteristics of early-stage Sertoli cells, offering a viable alternative to current models.
  • This iPSC-derived SLC model holds significant potential for advancing research in sex development and personalized reproductive medicine.