Related Experiment Video

Updated: Feb 2, 2026

Evaluation of the Spatial Distribution of γH2AX following Ionizing Radiation
09:28

Evaluation of the Spatial Distribution of γH2AX following Ionizing Radiation

Published on: August 7, 2010

13.2K

Effects of FOXM1 inhibition and ionizing radiation on melanoma cells

Vivienne S Lee1, Lucinda S McRobb1, Vaughan Moutrie2

  • 1Department of Clinical Medicine, Faculty of Medicine and Health Sciences, Macquarie University, Sydney, New South Wales 2109, Australia.

Oncology Letters
|November 9, 2018
PubMed

Insights

Siomycin A effectively reduces metastatic melanoma growth, death, and migration. However, combining this FOXM1 inhibitor with radiation therapy did not enhance treatment efficacy in this cell line.

Area of Science:

  • Oncology
  • Molecular Biology
  • Radiotherapy

Background:

  • Metastatic melanoma is often resistant to conventional treatments.
  • Forkhead box M1 (FOXM1) is a key regulator in cell proliferation and survival.
  • Targeted therapies combined with radiation show promise for improved efficacy.

Purpose of the Study:

  • To investigate the efficacy of Siomycin A (SIOA), a FOXM1 inhibitor, alone and in combination with radiation.
  • To assess the impact on growth, apoptosis, and migration in metastatic melanoma cells (SK-MEL-28).

Main Methods:

  • Utilized a metastatic melanoma cell line (SK-MEL-28) overexpressing FOXM1.
  • Administered Siomycin A (SIOA) at various concentrations.
  • Applied single doses of ionizing radiation (0-40 Gy).
  • Assessed apoptosis, proliferation, and migration (scratch wound assay).

Main Results:

  • SIOA alone potently inhibited melanoma cell proliferation and migration.
  • SIOA induced apoptosis at concentrations >1 µM, reducing FOXM1 and BCL2 protein levels.
  • Ionizing radiation inhibited growth and migration but did not significantly induce cell death.
  • SIOA pretreatment did not sensitize melanoma cells to radiation therapy.

Conclusions:

  • Siomycin A is an effective single agent for inducing cell death and inhibiting migration in metastatic melanoma cells with constitutive FOXM1 expression.
  • Combining Siomycin A with radiation therapy may not offer additional therapeutic benefits in this specific melanoma model.
Keywords:
FOXM1Siomycin Airradiationmelanomathiazole antibiotics

More Related Videos

Dual Effects of Melanoma Cell-derived Factors on Bone Marrow Adipocytes Differentiation07:00

Dual Effects of Melanoma Cell-derived Factors on Bone Marrow Adipocytes Differentiation

Published on: August 23, 2018

6.6K
Studying Normal Tissue Radiation Effects using Extracellular Matrix Hydrogels11:01

Studying Normal Tissue Radiation Effects using Extracellular Matrix Hydrogels

Published on: July 24, 2019

6.3K

Related Concept Videos

Biological Effects of Radiation02:59

Biological Effects of Radiation

All radioactive nuclides emit high-energy particles or electromagnetic waves. When this radiation encounters living cells, it can cause heating, break chemical bonds, or ionize molecules. The most serious biological damage results when these radioactive emissions fragment or ionize molecules. For example, α and β particles emitted from nuclear decay reactions possess much higher energies than ordinary chemical bond energies. When these particles strike and penetrate matter, they...
17.9K
Ionization Energy03:12

Ionization Energy

The amount of energy required to remove the most loosely bound electron from a gaseous atom in its ground state is called its first ionization energy (IE1). The first ionization energy for an element, X, is the energy required to form a cation with 1+ charge:
43.3K
Feedback Inhibition00:46

Feedback Inhibition

Biochemical reactions are occurring constantly in cells, converting starting substances to different products, usually with the help of enzymes that speed the reactions. Without enzymes, it would take far too long for most reactions to occur to be useful to the cell!
57.1K
Radiation: Applications01:17

Radiation: Applications

The average temperature of Earth is the subject of much current discussion. Earth is in radiative contact with both the Sun and dark space; it receives almost all its energy from the radiation of the Sun and reflects some of it into outer space. Dark space is very cold, about 3 K, so Earth radiates energy into it. For instance, heat transfer occurs from soil and grasses, the rate of which can be so rapid that frost can occur on clear summer evenings, even in warm latitudes.
The average...
1.8K
Buffer Effectiveness02:19

Buffer Effectiveness

Buffer solutions do not have an unlimited capacity to keep the pH relatively constant . Instead, the ability of a buffer solution to resist changes in pH relies on the presence of appreciable amounts of its conjugate weak acid-base pair. When enough strong acid or base is added to substantially lower the concentration of either member of the buffer pair, the buffering action within the solution is compromised.
The buffer capacity is the amount of acid or base that can be added to a given volume...
55.2K
Absorption of Radiation01:05

Absorption of Radiation

The rate of heat transfer by emitted radiation is described by the Stefan-Boltzmann law of radiation:
1.3K