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Updated: Feb 2, 2026

Methyl-binding DNA capture Sequencing for Patient Tissues
Published on: October 31, 2016
Investigations into the DNA-binding mode of doxorubicinone
Samuel Steucek Tartakoff1, Jennifer M Finan, Ellis J Curtis
1St. Lawrence University, Department of Chemistry, 23 Romoda Drive, Canton, New York 13617, USA. sglazier@stlawu.edu.
Abstract:
Cancer treatment is one of the major challenges facing the modern biomedical profession. Development of new small-molecule chemotherapeutics requires an understanding of the mechanism of action for these treatments, as well as the structure-activity relationship. Study of the well-known DNA-intercalating agent, doxorubicin, and its aglycone, doxorubicinone, was undertaken using a variety of spectroscopic and calorimetric techniques. It was found that, despite conservation of the planar, aromatic portion of doxorubicin, the agylcone does not intercalate; it instead likely binds to the DNA minor-groove.
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