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A method for direct quantification of the amount of DTPA in 111In monoclonal antibody preparations
International Journal of Radiation Applications and Instrumentation. Part A, Applied Radiation and Isotopes
|January 1, 1987
Abstract:
The bicyclic anhydride of DTPA-1-14C (BADTPA-1-14C) was synthesized and reacted with an antibody to human melanoma associated antigen (MAA) and with one to human class II major histocompatibility complex antigen (HLA-DR). DTPA-1-C incorporation per mole of anti MAA at molar ratios of 1:1 to 200:1 ranged from 0.5 to 25, while immunoreactivity ranged from 49 to 9%. With antibody to HLA-DR, results were similar. Anti MAA, but not anti HLA-DR, demonstrated polymerization upon conjugation. BADTPA-1-14C provides a convenient and accurate method for measuring the amount of DTPA in monoclonal antibody preparations and its effect on immunoreactivity.