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Related Experiment Videos

Sequence analysis of the gtfB gene from Streptococcus mutans.

T Shiroza, S Ueda, H K Kuramitsu

    Journal of Bacteriology
    |September 1, 1987
    PubMed
    Summary

    The Streptococcus mutans gtfB gene sequence was determined, revealing a gene encoding a large, hydrophilic glucosyltransferase I protein. This analysis also identified a potential downstream gene for another extracellular protein.

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    Area of Science:

    • Microbiology
    • Molecular Biology
    • Genetics

    Background:

    • Streptococcus mutans is a key cariogenic bacterium.
    • Glucosyltransferases (GTFs) play a crucial role in dental plaque formation.
    • Understanding GTF genes is vital for developing strategies against dental caries.

    Purpose of the Study:

    • To determine the nucleotide sequence of the gtfB gene from Streptococcus mutans GS-5.
    • To characterize the protein encoded by gtfB, including its structure and potential functions.
    • To investigate genes located downstream of gtfB.

    Main Methods:

    • Nucleotide sequencing of the gtfB gene.
    • Bioinformatic analysis of the deduced amino acid sequence.
    • Sequencing of the region downstream of the gtfB gene.

    Main Results:

    • The complete nucleotide sequence of the gtfB gene was determined.
    • The gtfB gene encodes a large (165,800 daltons), hydrophilic protein with a signal sequence and repeating units.
    • A putative gene for an extracellular protein, partially homologous to gtfB, was identified downstream.

    Conclusions:

    • The gtfB gene sequence provides insights into the structure of glucosyltransferase I.
    • The identified downstream gene suggests potential coordinated gene expression or related functions.
    • Further research on these genes could aid in understanding Streptococcus mutans virulence and developing anti-caries interventions.

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