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Height Resolution of Antibody Spots Measured by Spinning-Disk Interferometry on the BioCD
Kevin O'Brien1, Ming Zhao2, David Nolte3
1Department of Physics, University of California Berkeley, Berkeley, CA 94720-7300, USA. kpobrien@berkeley.edu.
Abstract:
Spinning-disc interferometry (SDI) is a high-speed laser scanning approach to surface metrology that uses common-path interferometry to measure protein spots on a BioCD disk. The measurement sensitivity depends on the scanning pitch and on the time-base. Based on high-resolution laser scanning images of printed antibody spots, we quantify the protein sensitivity as a function of the scan parameters. For smoothly printed antibody spots scanned with a transverse spatial resolution of 1 μm, the surface height precision for a single 100 μm diameter protein spot is approximately 1 pm. This detection sensitivity sets the fundamental limit of detection for label-free BioCD biosensors performing immunoassays.
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