Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Nuclear Fusion

Nuclear Fusion

The process of converting very light nuclei into heavier nuclei is also accompanied by the conversion of mass into large amounts of energy, a process called fusion. The principal source of energy in the sun is a net fusion reaction in which four hydrogen nuclei fuse and ultimately produce one helium nucleus and two positrons.
A helium nucleus has a mass that is 0.7% less than that of four hydrogen nuclei; this lost mass is converted into energy during the fusion. This reaction produces about...

Genome Annotation and Assembly

Genome Annotation and Assembly

The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.

Tagging and Fusion Proteins

Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...

SNAREs and Membrane Fusion

SNAREs and Membrane Fusion

Once a transport vesicle has recognized its target organelle, the vesicular membrane needs to fuse with the target membrane to unload the cargo. Transmembrane proteins called SNAREs present on organelle membranes and their vesicles, mediate vesicle fusion.
SNAREs exist in pairs that symmetrically interact and catalyze the fusion of the lipid bilayers in vesicle and target organelle. v-SNARE in the vesicle membrane are single polypeptide chains that bind to a complementary t-SNARE, composed of 2...

Gene Flow

Gene Flow

Gene flow is the transfer of genes among populations, resulting from either the dispersal of gametes or from the migration of individuals.

Gene Conversion

Gene Conversion

Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

FusionTarget: Computational framework for drug repurposing against modeled fusion protein structures from genomic breakpoints.

iScience·2026

Same author

How biological sex shapes differences in immune responses to infection.

Biology of sex differences·2026

Same author

RMzyme: regulations of RNA-modifying enzymes in humans.

Signal transduction and targeted therapy·2026

Same author

AllergenAI: a deep learning model predicting allergenicity based on protein sequence.

BMC bioinformatics·2025

Same author

Spatial2GWAS: a database for linking spatial transcriptomic regions with GWAS traits.

Nucleic acids research·2025

Same author

FusionPub, a therapeutic landscape of human fusion genes.

Briefings in bioinformatics·2025

Search research articles

Related Experiment Video

Updated: Feb 2, 2026

Annotation of Plant Gene Function via Combined Genomics, Metabolomics and Informatics

Annotation of Plant Gene Function via Combined Genomics, Metabolomics and Informatics

Published on: June 17, 2012

FusionGDB: fusion gene annotation DataBase.

Pora Kim¹, Xiaobo Zhou^1,2,3

¹Center for Computational Systems Medicine, School of Biomedical Informatics, The University of Texas Health Science Center at Houston, Houston, TX 77030, USA.

Nucleic Acids Research

|November 9, 2018

Summary

This summary is machine-generated.

FusionGDB is a new database that catalogs over 48,000 cancer gene fusions. This resource aids in understanding gene fusion functions and discovering new cancer biomarkers and therapies.

More Related Videos

The ITS2 Database

The ITS2 Database

Published on: March 12, 2012

Generation of Comprehensive Thoracic Oncology Database - Tool for Translational Research

Generation of Comprehensive Thoracic Oncology Database - Tool for Translational Research

Published on: January 22, 2011

Related Experiment Videos

Last Updated: Feb 2, 2026

Annotation of Plant Gene Function via Combined Genomics, Metabolomics and Informatics

Annotation of Plant Gene Function via Combined Genomics, Metabolomics and Informatics

Published on: June 17, 2012

The ITS2 Database

The ITS2 Database

Published on: March 12, 2012

Generation of Comprehensive Thoracic Oncology Database - Tool for Translational Research

Generation of Comprehensive Thoracic Oncology Database - Tool for Translational Research

Published on: January 22, 2011

Area of Science:

Genomics and Bioinformatics
Cancer Research
Molecular Biology

Background:

Gene fusions, resulting from chromosomal rearrangements, are key features of cancer genomes.
Numerous fusion genes (FGs) serve as critical biomarkers and therapeutic targets in various cancers.
Understanding FG function is crucial for advancing cancer diagnostics and treatment.

Purpose of the Study:

To establish FusionGDB, a comprehensive database for pan-cancer gene fusion annotation.
To facilitate the understanding of FG functions across different cancer types.
To promote the discovery of novel, clinically relevant fusion genes.

Main Methods:

Collected approximately 48,117 FGs from three major resources: ChiTaRS 3.1, TumorFusions, and TCGA.
Performed functional annotations, including open reading frame (ORF) assignment and protein feature retention analysis (39 features).
Generated fusion transcript and amino acid sequences considering multiple breakpoints and isoforms.

Main Results:

Identified 331 in-frame FGs retaining kinase domains, 303 retaining DNA-binding domains, and 667 retaining epigenetic factor domains.
Discovered 976 FGs with lost protein-protein interaction capabilities.
FusionGDB offers six annotation categories: FusionGeneSummary, FusionProtFeature, FusionGeneSequence, FusionGenePPI, RelatedDrug, and RelatedDisease.

Conclusions:

FusionGDB provides a valuable, integrated resource for exploring cancer gene fusions.
The database facilitates functional annotation and characterization of FGs.
FusionGDB supports research into FG-driven mechanisms and potential therapeutic strategies in oncology.