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Updated: Feb 2, 2026
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Cryopreservation of Preimplantation Embryos of Cattle, Sheep, and Goats
Published on: August 5, 2011
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Identification and characterization of ERV transcripts in goat embryos
Ruizhi Deng1, Chengquan Han1, Lu Zhao1
1College of Veterinary Medicine, Northwest A&F University, Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, Yangling, Shaanxi, China.
Reproduction (Cambridge, England)
|November 9, 2018
Summary
Endogenous retroviruses (ERVs) play a crucial role in early embryonic development. Specific ERV-derived transcripts, like lncRNA TCONS_00460156 and mRNA HSD17B11, are essential for goat embryonic genome activation and development.
Area of Science:
- Genomics
- Developmental Biology
- Molecular Biology
Background:
- Endogenous retroviruses (ERVs) are prevalent in mammalian genomes and influence gene expression.
- ERV expression is dynamic and stage-specific during early embryonic development.
- The precise roles of ERVs in regulating embryonic development are not fully understood.
Purpose of the Study:
- To investigate the contribution of ERVs to transcript regulation during embryonic genome activation (EGA) in goats.
- To identify specific ERV-derived transcripts involved in early embryonic development.
Main Methods:
- Construction of ERV and embryo RNA-seq libraries (IVF_2c and IVF_8c) from goat embryos.
- Gene Ontology (GO) and KEGG pathway analysis of ERV-associated genes.
- RNA sequencing, transcript identification, expression validation, and gene knockdown experiments.
Main Results:
- GO and KEGG analyses suggested ERV elements are linked to embryonic development.
- Identified three lncRNAs and one mRNA highly expressed during goat EGA.
- Knockdown of specific ERV-derived lncRNA (TCONS_00460156) and mRNA (HSD17B11) impaired embryo development.
Conclusions:
- Certain ERV-derived transcripts are essential for early goat embryonic development.
- Analyzing ERV expression profiles during EGA can elucidate their regulatory mechanisms in development.
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