Indirect pathway to pectoral fin motor neurons from nucleus ruber in the Nile tilapia Oreochromis niloticus

Supraspinal motor control systems of pectoral fins remain unclear in teleosts. Nucleus ruber of Goldstein (1905; NRg), which has been identified as the probable homologue of nucleus ruber of tetrapods, is a candidate structure serving for such functions. In the present study, we investigated possible involvement of the NRg in the control of pectoral fin movement by tract-tracing experiments in the Nile tilapia Oreochromis niloticus. Tracer injections into the NRg revealed the fiber course of rubrospinal tract. Rubrospinal fibers crossed the midline at the level of midbrain, descended through the tegmentum, and terminated in a region ventrally adjacent to the dorsal horn at the spinomedullary junction, without reaching the ventral horn where pectoral fin motor neurons are present. Tracer injection experiments into the dorsal horn region resulted in labeled terminals in proximities of presumed pectoral fin motor neurons in the ventral horn. Tracer injection experiments into the ventral horn resulted in retrogradely labeled neurons ventrally adjacent to the dorsal horn, where labeled terminals were detected following rubral injections. These anatomical analyses suggest that the NRg of actinopterygians is involved in the control of pectoral fin motor neurons through an indirect pathway via interneurons in the dorsal horn.