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Updated: Feb 2, 2026

Mass Spectrometry Analysis to Identify Ubiquitylation of EYFP-tagged CENP-A EYFP-CENP-A
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Malformations in the Murine Kidney Caused by Loss of CENP-F Function

Chanell O Haley1, Aoife M Waters2,3, D M Bader1

  • 1Division of Cardiovascular Medicine, Department of Medicine, Vanderbilt University, Nashville, Tennessee.

Anatomical Record (Hoboken, N.J. : 2007)
|November 9, 2018
PubMed

Insights

Loss of Centromere-binding protein F (CENP-F) causes significant kidney abnormalities in adult mice. This study reveals CENP-F

Area of Science:

  • Cell Biology
  • Developmental Biology
  • Nephrology

Background:

  • Centromere-binding protein F (CENP-F) is crucial for mitosis and interphase functions.
  • Previous research indicates CENP-F disruption negatively impacts human development.
  • Limited studies explore CENP-F's role in adult organ structure and function.

Purpose of the Study:

  • To investigate the consequences of CENP-F deletion on adult kidney structure and function.
  • To establish a link between CENP-F and kidney disease.

Main Methods:

  • Utilized a novel global knockout murine model lacking CENP-F.
  • Analyzed adult kidney structure and function in knockout mice.

Main Results:

  • Observed structural kidney abnormalities: loss of ciliary structure, tubule dilation, glomerular disruption.
  • Detected renal dysfunction, including hydronephrosis and acute kidney injury.
  • This study is the first to associate CENP-F with kidney disease.

Conclusions:

  • CENP-F deficiency leads to significant structural and functional kidney defects in adult mice.
  • The findings suggest CENP-F is essential for maintaining adult kidney homeostasis.
  • This research provides a foundation for further investigation into CENP-F's role in kidney disease pathogenesis.
Keywords:
CENP-Fkidney diseasemolecular genetics

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