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Related Concept Videos

Gene Therapy00:59

Gene Therapy

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Gene therapy is a technique where a gene is inserted into a person’s cells to prevent or treat a serious disease. The added gene may be a healthy version of the gene that is mutated in the patient, or it could be a different gene that inactivates or compensates for the patient’s disease-causing gene. For example, in patients with severe combined immunodeficiency (SCID) due to a mutation in the gene for the enzyme adenosine deaminase, a functioning version of the gene can be...
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Genome Size and the Evolution of New Genes03:21

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While every living organism has a genome of some kind (be it RNA, or DNA), there is considerable variation in the sizes of these blueprints. One major factor that impacts genome size is whether the organism is prokaryotic or eukaryotic. In prokaryotes, the genome contains little to no non-coding sequence, such that genes are tightly clustered in groups or operons sequentially along the chromosome. Conversely, the genes in eukaryotes are punctuated by long stretches of non-coding sequence.
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Modified Boxplots00:57

Modified Boxplots

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A standard box and whisker plot informs us about the spread of the data in a given sample. One can identify the minimum value, maximum value, first quartile value, second quartile or median value, and third quartile.
However, the box plot does not tell the reader about outliers - values that lie far from the center of the data. We can modify the standard box and whisker plot to identify the outliers and visualize the actual spread of the data in a sample.
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Gene Flow02:39

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Gene flow is the transfer of genes among populations, resulting from either the dispersal of gametes or from the migration of individuals.
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Gene Conversion02:08

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Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...
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Gene Families01:57

Gene Families

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Gene families consist of groups of genes proposed to have originated from a common ancestor. Typically these arise through events in which a gene or genes are mistakenly duplicated during cell division. Unlike their parent genes (which are subject to selection pressure to maintain function), these gene copies do not need to preserve their sequences and may evolve at a relatively faster rate.
Occasionally these regions can be adapted to take on new roles within the organism, becoming novel genes...
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Therapeutic Gene Delivery and Transfection in Human Pancreatic Cancer Cells using Epidermal Growth Factor Receptor-targeted Gelatin Nanoparticles
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Modified gelatin nanoparticles for gene delivery.

Osama Madkhali1, George Mekhail2, Shawn D Wettig3

  • 1School of Pharmacy, University of Waterloo, Waterloo ON, N2L 3G1, Canada; Department of Pharmaceutics, Faculty of Pharmacy, Jazan University, Jizan, Saudi Arabia.

International Journal of Pharmaceutics
|November 9, 2018
PubMed
Summary

Modified gelatin nanoparticles (GNPs) offer a promising, cost-effective, and biodegradable non-viral gene delivery system. Various modifications enhance transfection efficiency for superior gene therapy applications.

Keywords:
GelatinGene deliveryMethodModification

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Area of Science:

  • Biomaterials Science
  • Gene Therapy
  • Nanotechnology

Background:

  • Gelatin nanoparticles (GNPs) are widely utilized natural polymers for gene therapy.
  • Gelatin offers biocompatibility, biodegradability, and cost-effectiveness, making it ideal for gene delivery systems.

Purpose of the Study:

  • To review preparation methods for modified gelatin nanoparticles.
  • To evaluate the application of these modifications in gene delivery.
  • To highlight improvements in transfection and gene delivery efficiency.

Main Methods:

  • Review of literature on modified gelatin nanoparticle preparation.
  • Analysis of different modification strategies: cationic, PEGylated, thiolated, alendronate, and EGFR gelatin nanoparticles.
  • Assessment of the impact of modifications on gene delivery efficacy.

Main Results:

  • Various modifications significantly enhance the performance of gelatin nanoparticles for gene delivery.
  • Specific modifications like cationic, PEGylated, and thiolated gelatin show improved transfection rates.
  • EGFR and alendronate modifications offer targeted delivery advantages.

Conclusions:

  • Modified gelatin nanoparticles represent a versatile and efficient non-viral gene delivery platform.
  • Tailoring gelatin nanoparticle modifications can optimize gene delivery for various therapeutic applications.
  • These advancements hold significant potential for improving gene therapy outcomes.