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Updated: Feb 2, 2026

Interview: Glycolipid Antigen Presentation by CD1d and the Therapeutic Potential of NKT cell Activation
Published on: December 31, 2007
Structural determination of ananatoside A: An unprecedented 15-membered macrodilactone-containing glycolipid from
Charles Gauthier1, Serge Lavoie2, Marianne Piochon1
1INRS - Institut Armand-Frappier, Université du Québec, 531, boul. des Prairies, Laval, Québec, H7V 1B7, Canada.
Abstract:
The bacterium Pantoea ananatis was reported to produce glycolipid biosurfactants of unknown structures. Herein, we present the isolation and structural determination of ananatoside A, the main congener of a new family of 15-membered macrodilactone-containing glucolipids. The structure of ananatoside A was elucidated via chemical degradation and spectroscopic methods including 1D/2D NMR analysis, tandem MS/MS, GC-MS, HR-ESI-TOF-MS, MALDI-TOF-MS, and polarimetry. Computational methods were used to predict the most abundant conformers of ananatoside A.
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