Related Experiment Video

Updated: Feb 2, 2026

High-throughput Nitrobenzoxadiazole-labeled Cholesterol Efflux Assay
08:18

High-throughput Nitrobenzoxadiazole-labeled Cholesterol Efflux Assay

Published on: January 7, 2019

9.6K

The negative effect of ANGPTL8 on HDL-mediated cholesterol efflux capacity

Mengdie Luo1, Ziyu Zhang1, Yani Peng2

  • 1Department of Cardiovascular Medicine, The Second Xiangya Hospital, Central South University, No.139, Middle Renmin Road, Changsha, 410011, Hunan, China.

Cardiovascular Diabetology
|November 10, 2018
PubMed
Abstract

Insights

Angiopoietin-like protein 8 (ANGPTL8) negatively impacts high-density lipoprotein (HDL) function by reducing cholesterol efflux capacity. This finding suggests a novel pathway through which ANGPTL8 influences lipid metabolism and atherosclerosis progression.

Area of Science:

  • Lipid metabolism research
  • Cardiovascular disease mechanisms
  • Biomarker discovery

Background:

  • Angiopoietin-like protein 8 (ANGPTL8) is known to affect lipid metabolism by inhibiting lipoprotein lipase, increasing plasma triglyceride levels.
  • The precise mechanisms by which ANGPTL8 influences lipid metabolism, particularly its effects on high-density lipoprotein (HDL) function, remain incompletely understood.
  • HDL plays a crucial role in protecting against atherosclerosis progression, making its functional integrity a key area of research.

Purpose of the Study:

  • To investigate the impact of ANGPTL8 on the functional capacity of HDL.
  • To explore potential novel pathways through which ANGPTL8 affects lipid metabolism.
  • To determine the relationship between ANGPTL8 levels and HDL-mediated cholesterol efflux capacity in subjects with and without coronary artery disease (CAD) and diabetes mellitus (DM).

Main Methods:

  • Recruitment of 210 subjects for the study.
  • Quantification of plasma ANGPTL8 levels using enzyme-linked immunosorbent assays (ELISAs).
  • Measurement of cholesterol efflux capacity, a key biomarker of HDL function, using a H³-cholesterol loading THP-1 cell model.

Main Results:

  • Plasma ANGPTL8 levels were significantly higher in individuals with diabetes mellitus (DM) compared to those without DM.
  • Subjects with CAD and DM exhibited significantly reduced cholesterol efflux capacity compared to controls.
  • A significant inverse correlation was observed between plasma ANGPTL8 levels and cholesterol efflux capacity (r = -0.188, P < 0.01).
  • Regression analysis identified plasma ANGPTL8 as an independent contributor to reduced cholesterol efflux capacity (standardized β = -0.143, P = 0.023).

Conclusions:

  • ANGPTL8 exerts a negative influence on HDL-mediated cholesterol efflux capacity.
  • This finding suggests a novel mechanism by which ANGPTL8 contributes to dyslipidemia and potentially atherosclerosis.
  • Further research is warranted to elucidate the therapeutic implications of targeting ANGPTL8 for cardiovascular health.
Keywords:
ANGPTL8Cholesterol efflux capacityCoronary artery diseaseDiabetes

More Related Videos

Cholesterol Efflux Assay07:54

Cholesterol Efflux Assay

Published on: March 6, 2012

30.7K
Differential Effects of Lipid-lowering Drugs in Modulating Morphology of Cholesterol Particles09:15

Differential Effects of Lipid-lowering Drugs in Modulating Morphology of Cholesterol Particles

Published on: November 10, 2017

15.1K

Related Concept Videos

Lung Capacity01:47

Lung Capacity

The air in the lungs is measured in volumes and capacities. Lung volume measures reflect the amount of air taken in, released, or left over after a lung function, like a single inhalation. Lung capacity measures are sums of two or more lung volume measures.
56.3K
Receptor-mediated Endocytosis01:39

Receptor-mediated Endocytosis

Overview
110.9K
Cholesterol: Significance and Regulation01:29

Cholesterol: Significance and Regulation

Although not a source of energy, cholesterol plays a significant role as a foundational structure for bile salts, steroid hormones, and vitamin D, as well as being a crucial component of plasma membranes. Approximately 15% of blood cholesterol is derived from our diet, with the remainder synthesized from acetyl CoA by the liver and intestines. Cholesterol is eliminated from the body through its conversion into bile salts, which are eventually discarded in the feces.
Considering cholesterol and...
1.4K
Respiratory Capacities01:24

Respiratory Capacities

Respiratory capacities are crucial indicators of lung function, representing the maximum amount of air an individual's respiratory system can handle during various breathing phases.
One key metric is the Inspiratory Capacity (IC), which represents the maximum amount of air that can be inhaled with full effort. IC is calculated by summing the tidal volume and inspiratory reserve volume, typically ranging from 2.4 to 3.6 liters.
The Functional Residual Capacity (FRC) represents the air in the...
1.4K
Negative Regulator Molecules01:23

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
38.5K
Positive, Negative, and Zero Work00:58

Positive, Negative, and Zero Work

Work is done on an object when energy is transferred to the object. In other words, work is done when a force acts on a body that undergoes a displacement from one position to another. By definition, the work done by a force is the integral of the force with respect to the displacement along its path. Forces can vary as a function of position, and displacements can occur along various paths between two points. The magnitude of a force multiplied by the cosine of the angle that the force makes...
22.2K