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Updated: Feb 2, 2026

Gene Knock-in by CRISPR/Cas9 and Cell Sorting in Macrophage and T Cell Lines
Published on: November 13, 2021
Live imaging and tracking of genome regions in CRISPR/dCas9 knock-in mice
Jinzhi Duan1,2, Guangqing Lu1,2, Yu Hong3,2
1Graduate Program, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, 100730, China.
Abstract:
CRISPR/dCas9 is a versatile tool that can be used to recruit various effectors and fluorescent molecules to defined genome regions where it can modulate genetic and epigenetic markers, or track the chromatin dynamics in live cells. In vivo applications of CRISPR/dCas9 in animals have been challenged by delivery issues. We generate and characterize a mouse strain with dCas9-EGFP ubiquitously expressed in various tissues. Studying telomere dynamics in these animals reveals surprising results different from those observed in cultured cell lines. The CRISPR/dCas9 knock-in mice provide an important and versatile tool to mechanistically study genome functions in live animals.
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