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Related Experiment Video

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A DIE responsive NIR-fluorescent cell membrane probe.

Dan Wu1, Shane Cheung1, Gonzalo Sampedro1

  • 1Department of Chemistry, RCSI, 123 St Stephen's Green, Dublin 2, Ireland.

Biochimica Et Biophysica Acta. Biomembranes
|November 10, 2018
PubMed
Summary
This summary is machine-generated.

This study introduces a novel disaggregation-induced emission (DIE) probe for selective cell membrane imaging. The near-infrared probe switches on fluorescence upon disaggregation, enabling clear visualization of viable and apoptotic cells.

Keywords:
Disaggregation-induced emissionFixed cellLive cellNIR-fluorescence microscopyNIR-fluorophorePlasma membrane

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Area of Science:

  • Chemical Biology
  • Molecular Imaging
  • Biophysics

Background:

  • Achieving selective modulation of fluorophore emission in cellular environments is challenging.
  • Disaggregation-induced emission (DIE) offers a strategy for controllable fluorescence switching.
  • Amphiphilic probes are crucial for cellular imaging applications.

Purpose of the Study:

  • To develop and characterize a novel disaggregation-induced emission (DIE) probe for selective cell membrane imaging.
  • To investigate the aggregation/disaggregation properties of the probe in model systems and live cells.
  • To demonstrate the probe's utility in visualizing both viable and apoptotic cells.

Main Methods:

  • Synthesis and characterization of a bis-sulfonic acid substituted BF2-azadipyrromethene (NIR-AZA) probe.
  • Investigation of aggregation/disaggregation-induced emission in surfactant and liposomal systems.
  • Live and fixed cell imaging experiments using HeLa Kyoto and MC3T3 cell lines.

Main Results:

  • The NIR-AZA probe exhibits disaggregation-induced emission (DIE) with optical properties in the near-infrared spectrum.
  • Probe emission is responsive to aggregation state changes in model systems.
  • Rapid, selective plasma membrane staining of viable and apoptotic cells was achieved in real-time imaging.
  • Successful cell membrane staining was demonstrated in both live and formaldehyde-fixed cells.

Conclusions:

  • The developed NIR-AZA probe effectively utilizes DIE for selective and controllable cell membrane imaging.
  • The probe's unique bis-sulfonic acid substitution facilitates membrane localization without zwitterionic lipid substituents.
  • This work expands the applications of DIE responsive probes for cellular imaging and diagnostics.