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Updated: Feb 2, 2026

Measurement of Chitinase Activity in Biological Samples
Published on: August 22, 2019
Novel biologically active principles from spinach, goji and quinoa
Serena Fiorito1, Francesca Preziuso2, Francesco Epifano2
1Dipartimento di Scienze del Farmaco, Università degli Studi di Perugia, Via del Liceo, 06132 Perugia, Italy; Dipartimento di Farmacia, Università "G. d'Annunzio" Chieti-Pescara, Via dei Vestini 31, 66100 Chieti Scalo (CH), Italy.
Spinach, goji berries, and quinoa are rich sources of beneficial compounds. Microwave-assisted extraction using ethanol efficiently isolated key phytochemicals, highlighting their nutraceutical value.
Area of Science:
- Nutraceutical science
- Phytochemistry
- Food science
Background:
- Spinach, goji berries, and quinoa possess significant nutraceutical potential.
- These foods are rich in health-benefiting compounds like amino acids, fatty acids, carotenoids, and polyphenols.
Purpose of the Study:
- To evaluate the nutraceutical potential of spinach, goji berries, and quinoa.
- To quantify specific bioactive compounds using advanced analytical techniques.
Main Methods:
- Microwave-assisted extraction with various solvent mixtures (e.g., ethanol, hydroethanolic solutions).
- Ultra-high-performance liquid chromatography (UHPLC) with photodiode array detection for compound quantification.
- Analysis of compounds including 7-isopentenyloxycoumarin, auraptene, umbelliprenin, boropinic acid, and 4'-geranyloxyferulic acid.
Main Results:
- Ethanol proved to be the most effective solvent for extraction, yielding higher amounts of target compounds.
- Concentrations of identified phytochemicals ranged from 2.01 to 49.22 µg/g of dry extract.
- Spinach, goji, and quinoa were confirmed as valuable sources of oxyprenylated umbelliferone and ferulic acid derivatives.
Conclusions:
- Spinach, goji berries, and quinoa are excellent sources of nutraceutical compounds.
- Microwave-assisted extraction with ethanol is an efficient method for isolating these valuable phytochemicals.
- The study validates the health benefits associated with these plant-based foods.
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