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Published on: July 15, 2019
Biodegradation of deoxynivalenol and its derivatives by Devosia insulae A16
Gang Wang1, Yanxia Wang1, Fang Ji1
1Institute of Food Safety and Nutrition, Jiangsu Academy of Agricultural Sciences/Key Lab of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base/Key Laboratory of Control Technology and Standard for Agro-Product Quality and Safety, Ministry of Agriculture/Key Laboratory of Agro-Product Safety Risk Evaluation, Ministry of Agriculture/Collaborative Innovation Center for Modern Grain Circulation and Safety, Nanjing 210014, China.
Abstract:
Deoxynivalenol (DON), a notorious mycotoxin mainly found in Fusarium-contaminated crops, causes great loss in livestock farming and severe safety risks to human health. Here we report the isolation of a Gram-negative bacterial strain with effective biodegrading abilities on DON and its derivatives including 3-acetyl-DON and 15-acetyl-DON. The strain was identified as Devosia insulae A16 on the basis of morphological and physiological characteristics and 16S rRNA-based phylogenetic analysis. D. insulae A16 was able to degrade 88% of 20 mg/l DON within 48 h under aerobic conditions at 35 °C and neutral pH. The major degradation product of DON and its derivatives was 3-keto-DON by the oxidation of the hydroxyl group at C-3. Both 3-acetyl-DON and 15-acetyl-DON underwent a deacetylation reaction to generate DON prior to the degradation to 3-keto-DON. The results provide the potential use of D. insulae A16 as a biodegradation agent to control DON contamination in cereals.
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