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Exosome-transmitted LINC00461 promotes multiple myeloma cell proliferation and suppresses apoptosis by modulating

Mingyang Deng1, Huan Yuan1, Sufang Liu1

  • 1Department of Hematology, The Second Xiangya Hospital, Central South University, Changsha, China.

Cytotherapy
|November 10, 2018
PubMed
Abstract

Insights

Long noncoding RNA LINC00461 promotes multiple myeloma (MM) cell growth by sponging miR-15a/16. Mesenchymal stromal cell-derived exosomes enhance MM proliferation via LINC00461, suggesting therapeutic potential.

Area of Science:

  • Oncology
  • Molecular Biology
  • Gene Regulation

Background:

  • Multiple myeloma (MM) is a plasma cell malignancy with incompletely understood developmental mechanisms.
  • Long noncoding RNAs (lncRNAs) are increasingly recognized for their roles in regulating MM pathogenesis.

Purpose of the Study:

  • To investigate the role of LINC00461 in multiple myeloma (MM) development.
  • To explore the functional relationship between LINC00461, microRNAs, and MM cell behavior.
  • To determine the involvement of mesenchymal stromal cell (MSC)-derived exosomes in MM progression via LINC00461.

Main Methods:

  • Quantified LINC00461 expression in MM patient samples using real-time PCR.
  • Utilized short hairpin RNAs (shRNAs) to knockdown LINC00461 in MM cell lines.
  • Assessed the impact of LINC00461 knockdown on MM cell proliferation and apoptosis.
  • Analyzed the function of MSC-derived exosomes in MM using chamber assays.

Main Results:

  • LINC00461 expression was significantly elevated in MM.
  • LINC00461 knockdown markedly inhibited MM cell proliferation and induced apoptosis.
  • LINC00461 was found to relieve the suppressive effect of miR-15a/miR-16 on BCL-2 expression.
  • MSC-derived exosomes promoted MM cell proliferation, mediated by LINC00461.

Conclusions:

  • LINC00461 acts as a molecular sponge for miR-15a/16.
  • Highly expressed in MSC-derived exosomes, LINC00461 enhances MM cell proliferation.
  • LINC00461 presents a promising therapeutic target for multiple myeloma.
Keywords:
BCL-2LINC00461exosomemicroRNA-15a/16multiple myeloma

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