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Silencing of Xeroderma Pigmentosum Group D Gene Promotes Hepatoma Cell Growth by Reducing P53 Expression

Hao Ding1, Zhili Wen1, Guofang Sun2

  • 1Department of Gastroenterology, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, China (mainland).

Medical Science Monitor : International Medical Journal of Experimental and Clinical Research
|November 10, 2018
PubMed

Insights

Silencing the xeroderma pigmentosum group D (XPD) gene in hepatoma cells promoted cell proliferation and invasion by reducing P53 expression. This suggests XPD plays a role in regulating hepatoma cell growth.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Hepatoma, a primary liver cancer, poses significant health challenges.
  • Understanding the molecular mechanisms driving hepatoma growth is crucial for developing effective therapies.
  • The role of xeroderma pigmentosum group D (XPD) in hepatoma progression requires further investigation.

Purpose of the Study:

  • To investigate the effect of XPD gene silencing on hepatoma cell growth.
  • To elucidate the underlying molecular mechanisms, particularly the involvement of P53 signaling.

Main Methods:

  • XPD gene silencing was achieved using small interfering RNA (siRNA) in hepatoma cells.
  • Cell viability was assessed using MTT assays.
  • Apoptosis was detected by TUNEL assay and flow cytometry.
  • Invasive ability was evaluated using Transwell assays.
  • Gene and protein expression levels of key regulators (Mdm2, Mdm4, CyclinD1, P21, Bax, P53, C-sis, Bcl-2) were analyzed via real-time PCR and Western blotting.

Main Results:

  • XPD siRNA significantly reduced XPD expression at both mRNA and protein levels.
  • XPD silencing promoted hepatoma cell proliferation, reduced apoptosis, and enhanced invasive ability.
  • Silencing XPD led to increased expression of CyclinD1, Bcl-2, and C-sis, while decreasing P21, Mdm2, Mdm4, Bax, and P53.
  • Inhibition of P53 further amplified the effects of XPD silencing on hepatoma cell growth.

Conclusions:

  • XPD silencing promotes hepatoma cell growth.
  • The mechanism involves the downregulation of P53 expression.
  • Targeting XPD could be a potential therapeutic strategy for hepatoma, warranting further research.

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