Author Correction: Necroptosis microenvironment directs lineage commitment in liver cancer
Marco Seehawer1,2, Florian Heinzmann1,2, Luana D'Artista1,2
1Department of Internal Medicine VIII, University Hospital Tuebingen, Tuebingen, Germany.
Nature
|November 10, 2018
Summary
This study details corrections to genetic constructs used in research. Specifically, the pCaMIN construct now uses mouse NrasG12V, and the pCAMIA construct uses human AKT1 for improved accuracy.
Area of Science:
- Molecular Biology
- Genetics
- Biotechnology
Background:
- Accurate genetic constructs are crucial for reproducible biological research.
- Previous versions of pCaMIN and pCAMIA constructs contained species discrepancies.
Purpose of the Study:
- To correct and clarify the genetic components of the pCaMIN and pCAMIA constructs.
- To ensure the scientific accuracy of published research involving these constructs.
Main Methods:
- Comparative analysis of construct formulations.
- Correction of gene sequences for mouse MYC, mouse NrasG12V, and human AKT1.
Main Results:
- The pCaMIN construct was revised to include mouse MYC and mouse NrasG12V.
- The pCAMIA construct was revised to include mouse Myc and human AKT1.
Conclusions:
- The corrected constructs enhance the precision of molecular biology studies.
- These updates ensure greater reliability in research utilizing these specific genetic tools.
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