Related Experiment Video
Updated: Feb 2, 2026

Flavonoid Content During the Growth and Floral Development of Calendula officinalis L.
Published on: June 27, 2025
[Effect of anti-inflammatory flavonoid chrysin on osteogenesis of preosteoblast MC3T3-E1 cells]
1Department of Stomatology, Hangzhou Red Cross Hospital. Hangzhou 310000, Zhejiang Province, China.
Purpose:
To investigation the effect of flavonoid chrysin on osteogenesis of preosteoblast MC3T3-E1 cells.
Methods:
After evaluation of the toxic effect of chrysin, preosteoblast MC3T3-E1 cell suspension was prepared with DMEM and then the cells were cultured with 0 and 25 μmol/L chrysin. Real-time quantitative PCR was used to detect the expression of osteogenic marlcers including Runx2, ColA1 and OCN. Statistical analysis was performed using SPSS17.0 software package.
Results:
25 μmol/L chrysin had no toxic effect on preosteoblast MC3T3-E1 cells. After co-culture with 25 μmol/L chrysin,the expression of Runx2, ColA1 and OCN was highest at the 7th,14th and 21th day,respectively.
Conclusions:
25 μmol/L chrysin can promote osteogenic gene expression of preosteoblast MC3T3-E1 cell.
Related Concept Videos
E1 Reaction: Kinetics and Mechanism
E1 Reaction: Stereochemistry and Regiochemistry
Inflammatory Response
Inflammation can be triggered by various stimuli, such as impact, abrasion, chemical irritation, infections, and extreme hot or cold temperatures. These can damage cells and connective tissue fibers,...
Inflammatory Response II: Inflammatory Exudate and Tissue Repair
The typical wound exudate is odorless, transparent, straw-colored, thin, and watery. Exudate, however, can differ depending on the state of wound healing. Likewise, the...
Antiasthma Drugs: Mast Cell Stabilizers and Anti-IgE Drugs
Mast cell stabilizers, such as cromolyn (also known as sodium cromoglycate) and nedocromil (Tilade), are effective drugs in asthma management. These stabilizers hinder histamine release by skillfully obstructing the activation of mast cells and other cellular entities. Notably, they navigate this task without...
Inflammatory Response I: Vascular and Cellular

