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Updated: Feb 2, 2026

A Microplate Assay to Assess Chemical Effects on RBL-2H3 Mast Cell Degranulation: Effects of Triclosan without Use of an Organic Solvent
Published on: November 1, 2013
[Combined effects of fluoxetine and triclosan on Pseudorasbora parva.]
Liang Yan1,2, Lei Mu1, Hong Xing Chen3
1Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China.
Abstract:
We examined the effects of binary mixture of two different pharmaceutical and personal care products (PPCPs) on aquatic organisms. The topmouth gudgeon Pseudorasbora parva, native to northeast China, was used to study the combined effects of fluoxetine (FLX) and triclosan (TCS) on its different organs. After acute (4 h) and chronic (42 d) treatments, the combined effects of FLX/TCS on phase I and phase II detoxification enzymes, nervous system, digestive system and antioxidant system were evaluated. The results showed that the AChE activity in the brain of P. parva was transiently inhibited, whereas the activity of EROD in the liver was continuously inhi-bited. The activity of α-Glu in the intestine was induced after acute exposure but was inhibited after chronic exposure. Meanwhile, the level of lipid peroxidation in the liver was elevated after chronic exposure. In conclusion, the mixture of fluoxetine and triclosan produced toxic effects on multiple organs of P. parva, which could be alleviated after prolonged exposure, indicating an acclimation. However, the mechanisms of this acclimation needed further study.
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